The Role of Cryptochrome 2 in Flowering in Arabidopsis1
The Role of Cryptochrome 2 in Flowering in Arabidopsis1
复制标题
隐花色素 2 在拟南芥开花中的作用
作者：
Salah El;C. Alonso‐Blanco;A. Peeters;C. Wagemaker;J. Weller;M. Koornneef
我们研究了cry2与各种开花途径之间的遗传互作，以及光周期和春化对开花的调控。为此，我们结合了CRY2的三个等位基因，野生型CRY2- landsberg erecta (Ler)， CRY2功能缺失的零等位基因，以及功能获得的CRY2- cape Verde Islands (Cvi)，突变体代表了各种光感受器和开花途径。CRY2等位基因与光感受器突变体的结合分析表明，CRY2- cvi可以弥补phyA和cry1的缺失，也表明CRY2不需要功能性的phyA或cry1。光周期途径突变体分析显示co和gi对CRY2等位基因的上位性，表明CRY2需要co和gi基因的产物来促进开花。与Ler相比，该途径的所有双突变体的光周期响应都大大降低。相比之下，自主通路基因的突变是加在CRY2等位基因上的，部分克服了CRY2- cvi的影响，恢复了日长响应性。这3个CRY2等位基因与fr - sf2和/或FLC-Sf2基因组合时，对日长敏感，当春化去除fr - sf2和FLC-Sf2等位基因引起的延迟开花后，这种敏感性可以恢复。此外，我们观察了FLC和CRY2基因的表达，发现CRY2受FLC的负调控。这些结果表明，光周期和flc依赖的上游途径之间存在相互作用，这两种途径的共同下游目标是SOC1和FT。
We have investigated the genetic interactions between cry2 and the various flowering pathways in relation to the regulation of flowering by photoperiod and vernalization. For this, we combined three alleles of CRY2, the wild-type CRY2-Landsberg erecta (Ler), a cry2 loss-of-function null allele, and the gain-of-function CRY2-Cape Verde Islands (Cvi), with mutants representing the various photoreceptors and flowering pathways. The analysis of CRY2 alleles combined with photoreceptor mutants showed that CRY2-Cvi could compensate the loss of phyA and cry1, also indicating that cry2 does not require functional phyA or cry1. The analysis of mutants of the photoperiod pathway showed epistasis of co and gi to the CRY2 alleles, indicating that cry2 needs the product of CO and GI genes to promote flowering. All double mutants of this pathway showed a photoperiod response very much reduced compared with Ler. In contrast, mutations in the autonomous pathway genes were additive to the CRY2 alleles, partially overcoming the effects of CRY2-Cvi and restoring day length responsiveness. The three CRY2 alleles were day length sensitive when combined with FRI-Sf2 and/or FLC-Sf2 genes, which could be reverted when the delay of flowering caused by FRI-Sf2 and FLC-Sf2 alleles was removed by vernalization. In addition, we looked at the expression of FLC and CRY2 genes and showed that CRY2 is negatively regulated by FLC. These results indicate an interaction between the photoperiod and the FLC-dependent pathways upstream to the common downstream targets of both pathways, SOC1 and FT.
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