Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2).
Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2).
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DOI：
10.3390/cells9122670
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发表时间：
2020-12-11
期刊：
影响因子：
6
通讯作者：
Sasaki H
中科院分区：
文献类型：
--
作者：
Yamamoto N;Takeda S;Hatsusaka N;Hiramatsu N;Nagai N;Deguchi S;Nakazawa Y;Takata T;Kodera S;Hirata A;Kubo E;Sasaki H
据观察，热带和亚热带地区居民的核性白内障患病率明显高于温带和亚北极地区。我们假设环境温度升高可能会导致核性白内障的发生。我们的计算机模拟结果显示，在温带和热带地区，人类透镜温度范围为35.0 °C至37.5 °C，具体取决于环境温度。使用连接到温度计的传感器仔细监测细胞培养实验中定期更换期间的培养基温度变化，并且当将热板装置的温度分别设定在35.0 °C、37.5 °C、40.0 °C和42.5 °C时，5分钟后显示出1.9 °C、3.0 °C、1.7 °C和0.1 °C的降低。在新创建的永生化人透镜上皮细胞系克隆NY 2（iHLEC-NY 2）中，与35.0 °C相比，在37.5 °C培养温度下，αA晶体蛋白的RNA合成量、蛋白质表达量和分泌到培养基中的淀粉样蛋白β（Aβ）1-40的量增加。在短期培养实验中，在白内障中观察到的Aβ1-40的分泌在37.5 °C下比35.0 °C下增加，这表明长期暴露于高温环境可能会增加白内障的风险。
The prevalence of nuclear cataracts was observed to be significantly higher among residents of tropical and subtropical regions compared to those of temperate and subarctic regions. We hypothesized that elevated environmental temperatures may pose a risk of nuclear cataract development. The results of our in silico simulation revealed that in temperate and tropical regions, the human lens temperature ranges from 35.0 °C to 37.5 °C depending on the environmental temperature. The medium temperature changes during the replacement regularly in the cell culture experiment were carefully monitored using a sensor connected to a thermometer and showed a decrease of 1.9 °C, 3.0 °C, 1.7 °C, and 0.1 °C, after 5 min when setting the temperature of the heat plate device at 35.0 °C, 37.5 °C, 40.0 °C, and 42.5 °C, respectively. In the newly created immortalized human lens epithelial cell line clone NY2 (iHLEC-NY2), the amounts of RNA synthesis of αA crystallin, protein expression, and amyloid β (Aβ)1-40 secreted into the medium were increased at the culture temperature of 37.5 °C compared to 35.0 °C. In short-term culture experiments, the secretion of Aβ1-40 observed in cataracts was increased at 37.5 °C compared to 35.0 °C, suggesting that the long-term exposure to a high-temperature environment may increase the risk of cataracts.
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影响因子：
3.4
作者：
Ibaraki, N;Chen, SC;Reddy, VN
通讯作者：
Reddy, VN
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56.9
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作者：
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