The catalytic subunit of cAMP-dependent protein kinase from Ascaris suum. The cloning and structure of a novel subtype of protein kinase A.
The catalytic subunit of cAMP-dependent protein kinase from Ascaris suum. The cloning and structure of a novel subtype of protein kinase A.
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来自猪蛔虫的 cAMP 依赖性蛋白激酶的催化亚基。
DOI：
10.1111/j.1432-1033.1995.tb20788.x
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
H. Hofer
中科院分区：
文献类型：
--
作者：
S. Jung;R. Hoffmann;P. Rodríguez;R. Mutzel;H. Hofer
A complete cDNA clone encoding the catalytic subunit of cAMP-dependent protein kinase of Ascaris suum was constructed from two overlapping partial clones. The encoded sequence of 337 amino acids is 48% identical with the sequence of mouse C alpha subunit. Approximately the same low similarity was found with the sequence of the C subunit from another nematode, Caenorhabditis elegans. The N-terminal 14 amino acids and the myristoylation site of the mammalian protein are not contained in the enzyme from Ascaris. Two cysteines (Cys33 and Cys319) replace a basic residue in the N-terminal region and an acidic amino acid near the C-terminus which are conserved in all known C subunits from other sources. The substitutions provide the possibility of disulfide bridge formation between the N-terminal and C-terminal parts of the protein. There is strong evidence that a single gene encodes cAMP-dependent protein kinase in Ascaris. Modelling of the sequence into the coordinates of the X-ray structure of the mammalian enzyme suggest a high degree of conservation in the three-dimensional structure. However, structural variations occur at the surface of the protein near the catalytic cleft and are likely to account for the variations in substrate specificity previously observed between the purified protein kinase from Ascaris [Thalhofer, H. P., Daum, G., Harris, B. G. & Hofer, H. W. (1988) J. Biol. Chem. 263, 952-957] and the mammalian enzyme.
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猪蛔虫肌肉中环状 3,5-AMP 结合蛋白的纯化和特性。
影响因子：
3.9
作者：
Thalhofer,HP;Starz,W;Daum,G;Wurster,B;Harris,BG;Hofer,HW
通讯作者：
Hofer,HW
保守的螺旋基序补充了蛋白激酶核心。
DOI：
10.1073/pnas.90.22.10618
发表时间：
1993
影响因子：
11.1
作者：
Veron,M;Radzio-Andzelm,E;Tsigelny,I;TenEyck,LF;Taylor,SS
通讯作者：
Taylor,SS
秀丽隐杆线虫 cAMP 依赖性蛋白激酶催化亚基基因的克隆、表征和表达。
DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Gross,RE;Bagchi,S;Lu,X;Rubin,CS
通讯作者：
Rubin,CS
编码犬钩虫第三阶段感染性幼虫 cAMP 依赖性蛋白激酶催化亚基的 cDNA 的克隆和表征。
影响因子：
1.5
作者：
Hawdon,JM;Jones,BF;Hotez,PJ
通讯作者：
Hotez,PJ
DOI：
10.1073/pnas.83.5.1300
发表时间：
1986-03
影响因子：
11.1
作者：
Michael D. Uhler;D. Carmichael;D. C. Lee;John C. Chrivia;E. Krebs;G. McKnight
通讯作者：
Michael D. Uhler;D. Carmichael;D. C. Lee;John C. Chrivia;E. Krebs;G. McKnight