Unique microbial communities persist in individual cystic fibrosis patients throughout a clinical exacerbation.
Unique microbial communities persist in individual cystic fibrosis patients throughout a clinical exacerbation.
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DOI：
10.1186/2049-2618-1-27
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发表时间：
2013-11-01
期刊：
影响因子：
15.5
通讯作者：
O'Toole GA
中科院分区：
文献类型：
--
作者：
Price KE;Hampton TH;Gifford AH;Dolben EL;Hogan DA;Morrison HG;Sogin ML;O'Toole GA
囊性纤维化(CF)是由囊性纤维化跨膜传导调节基因的遗传性突变引起的，并导致高度有利于多菌感染的肺环境。在一生中，细菌多样性的减少和肺部铜绿假单胞菌的存在与肺部疾病的恶化有关。然而，到目前为止，社区多样性、总体微生物负荷或单个微生物的变化还没有显示出与CF患者急性加重的开始相关。我们对17例成人CF患者在整个临床加重、治疗和康复过程中进行了跟踪，使用深度测序和定量PCR对自发咳痰标本进行了特征分析。我们鉴定了大约170个细菌属，其中12个细菌占所有患者样本总细菌载量的90%以上。在任何单个患者样本中丰富的属往往在大多数样本中都能被检测到。我们发现，铜绿假单胞菌的绝对载菌量、绝对细菌载量或检测到的任何单个属的相对丰度或群落多样性不能区分临床分期。相反，我们发现每个患者痰微生物群的微生物结构都是不同的，对病情恶化和抗生素治疗具有弹性。与以前报道的痰微生物组研究一致，我们发现，无论临床状态如何，种群水平上的属的总丰度和相对丰度对于个体患者都是显着稳定的。对每一个属的多样性和相对丰度的逐个病人分析揭示了复杂的微生物图景，并突出了确定恶化的普遍微生物特征的难度。总体而言，在属的水平上，我们没有发现临床阶段的微生物特征的证据。
Cystic fibrosis (CF) is caused by inherited mutations in the cystic fibrosis transmembrane conductance regulator gene and results in a lung environment that is highly conducive to polymicrobial infection. Over a lifetime, decreasing bacterial diversity and the presence of Pseudomonas aeruginosa in the lung are correlated with worsening lung disease. However, to date, no change in community diversity, overall microbial load or individual microbes has been shown to correlate with the onset of an acute exacerbation in CF patients. We followed 17 adult CF patients throughout the course of clinical exacerbation, treatment and recovery, using deep sequencing and quantitative PCR to characterize spontaneously expectorated sputum samples We identified approximately 170 bacterial genera, 12 of which accounted for over 90% of the total bacterial load across all patient samples. Genera abundant in any single patient sample tended to be detectable in most samples. We found that clinical stages could not be distinguished by absolute Pseudomonas aeruginosa load, absolute total bacterial load or the relative abundance of any individual genus detected, or community diversity. Instead, we found that the microbial structure of each patient’s sputum microbiome was distinct and resilient to exacerbation and antibiotic treatment. Consistent with previously reported sputum microbiome studies we found that total and relative abundance of genera at the population level were remarkably stable for individual patients regardless of clinical status. Patient-by-patient analysis of diversity and relative abundance of each individual genus revealed a complex microbial landscape and highlighted the difficulty of identifying a universal microbial signature of exacerbation. Overall, at the genus level, we find no evidence of a microbial signature of clinical stage.
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影响因子：
3.7
作者：
Zemanick ET;Harris JK;Wagner BD;Robertson CE;Sagel SD;Stevens MJ;Accurso FJ;Laguna TA
通讯作者：
Laguna TA
影响因子：
3.7
作者：
Zemanick, Edith T.;Wagner, Brandie D.;Harris, J. Kirk
通讯作者：
Harris, J. Kirk
影响因子：
9.4
作者：
Horz, HP;Vianna, ME;Conrads, G
通讯作者：
Conrads, G
DOI：
10.1038/ismej.2010.175
发表时间：
2011-05
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
肺部恶化抗生素治疗对囊性纤维化细菌多样性的影响
影响因子：
5.2
作者：
Daniels, T. W. V.;Rogers, G. B.;Carroll, M. P.
通讯作者：
Carroll, M. P.