Partitioning core and satellite taxa from within cystic fibrosis lung bacterial communities.
Partitioning core and satellite taxa from within cystic fibrosis lung bacterial communities.
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DOI：
10.1038/ismej.2010.175
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发表时间：
2011-05
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影响因子：
--
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中科院分区：
文献类型：
--
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囊性纤维化（CF）患者患有慢性细菌性肺部感染，在大多数情况下导致死亡。维持这些患者肺功能的需要意味着表征这些感染至关重要。越来越多的非培养分析正在扩大与CF呼吸道样本相关的细菌种类的数量;然而，这些种类的潜在意义尚不清楚。在这里，我们应用生态统计工具，这样的文化独立的数据，以一种新的方式，分区类群内的元亲核性的核心和卫星物种。从14例临床稳定的成人CF患者中获得痰液和临床数据。构建了35属82个分类单元的14个rRNA基因文库，共鉴定了2139个细菌克隆。Shannon-Wiener和类群丰富度分析证实，细菌多样性没有采样不足。利用方差与平均多度的比值对物种多度分布进行分解，将物种多度分布客观地划分为核心种和卫星种。卫星群由33属67个细菌分类单元组成，核心群由7属15个细菌分类单元组成，其中假单胞菌属1个，链球菌属2个，奈瑟氏菌属2个，卡托氏菌属1个，紫单胞菌属1个，普雷沃氏菌属5个，韦荣氏菌属3个，最后4个为厌氧菌。核心菌群以铜绿假单胞菌为主。其他公认的CF病原体是罕见的。Mantel和部分Mantel试验评估了哪些临床因素影响观察到的组成。CF跨膜传导调节基因型和抗生素治疗与所有核心类群相关。肺功能与丰富度相关。这些核心和卫星物种的结果在CF肺的临床意义进行了讨论。
Cystic fibrosis (CF) patients suffer from chronic bacterial lung infections that lead to death in the majority of cases. The need to maintain lung function in these patients means that characterising these infections is vital. Increasingly, culture-independent analyses are expanding the number of bacterial species associated with CF respiratory samples; however, the potential significance of these species is not known. Here, we applied ecological statistical tools to such culture-independent data, in a novel manner, to partition taxa within the metacommunity into core and satellite species. Sputa and clinical data were obtained from 14 clinically stable adult CF patients. Fourteen rRNA gene libraries were constructed with 35 genera and 82 taxa, identified in 2139 bacterial clones. Shannon–Wiener and taxa-richness analyses confirmed no undersampling of bacterial diversity. By decomposing the distribution using the ratio of variance to the mean taxon abundance, we partitioned objectively the species abundance distribution into core and satellite species. The satellite group comprised 67 bacterial taxa from 33 genera and the core group, 15 taxa from 7 genera (including Pseudomonas (1 taxon), Streptococcus (2), Neisseria (2), Catonella (1), Porphyromonas (1), Prevotella (5) and Veillonella (3)], the last four being anaerobes). The core group was dominated by Pseudomonas aeruginosa. Other recognised CF pathogens were rare. Mantel and partial Mantel tests assessed which clinical factors influenced the composition observed. CF transmembrane conductance regulator genotype and antibiotic treatment correlated with all core taxa. Lung function correlated with richness. The clinical significance of these core and satellite species findings in the CF lung is discussed.
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影响因子：
14.9
作者：
Ludwig, W;Strunk, O;Schleifer, KH
通讯作者：
Schleifer, KH
影响因子：
4.8
作者：
Fisher, RA;Corbet, AS;Williams, CB
通讯作者：
Williams, CB
影响因子：
7
作者：
Nasidze, Ivan;Li, Jing;Stoneking, Mark
通讯作者：
Stoneking, Mark
影响因子：
4.4
作者：
Ashelford, KE;Chuzhanova, NA;Weightman, AJ
通讯作者：
Weightman, AJ
影响因子：
4.4
作者：
Ashelford, Kevin E.;Chuzhanova, Nadia A.;Weightman, Andrew J.
通讯作者：
Weightman, Andrew J.