Electrochemical immunosensors for antibodies to peanut allergen ara h2 using gold nanoparticle-peptide films.
Electrochemical immunosensors for antibodies to peanut allergen ara h2 using gold nanoparticle-peptide films.
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DOI：
10.1021/ac101110q
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发表时间：
2010-07-01
影响因子：
7.4
通讯作者：
Rusling, James F.
中科院分区：
文献类型：
--
作者：
Liu, Hongyun;Malhotra, Ruchika;Peczuh, Mark W.;Rusling, James F.
对花生和坚果的危及生命的过敏可以通过检测患者血清中过敏原的抗体（IgE）来发现。在此，我们比较了几种免疫传感器样的方法灵敏检测的抗体的肽序列的主要花生过敏原，花生hypogaea 2（阿拉H2）。该传感器的特点是来自Ara h2的主要IgE结合表位的合成肽层附着在热解石墨（PG）电极上的致密金纳米颗粒（AuNP）膜上。将金纳米粒子-肽传感器用于检测血清中的鸡抗花生抗体（IgY）。将法拉第和非法拉第阻抗策略与安培检测进行比较。采用辣根过氧化物酶（HRP）标记的山羊抗鸡二抗（Ab 2）与传感器上的IgY结合并提供放大信号。最好的阻抗传感器配置功能的酶产物的HPR催化沉淀到传感器上的非法拉第阻抗测量。该传感器配置对于未稀释小牛血清中的IgY抗体具有5 pg mL−1的最佳检测限（DL）和超过5个数量级（5 pg mL−1至1 µg mL−1）的最佳线性范围。该DL比无标记阻抗免疫传感器（0.5 ng mL−1）低100倍，比在电流免疫传感器中使用HRP-Ab 2时（0.3 ng mL−1）低60倍。
Life-threatening allergies to peanuts and tree nuts can be revealed by detecting antibodies (IgEs) to their allergens in patient serum. Herein, we compare several immunosensor-like methodologies for sensitive detection of antibodies to a peptide sequence from the major peanut allergen, Arachis hypogaea 2 (Ara h2). The sensors feature a synthetic peptide layer of the major IgE-binding epitope from Ara h2 attached to a dense gold nanoparticle (AuNP) film on a pyrolytic graphite (PG) electrode. The AuNP-peptide sensor was used to determine model chicken anti-peanut antibodies (IgY) in serum. Faradaic and non-Faradaic impedance strategies were compared to amperometric detection. Measurements employed goat anti-chicken secondary antibodies (Ab2) labeled with horseradish peroxidase (HRP) to bind to IgY on the sensor and provide amplified signals. The best impedimetric sensor configuration featured HPR-catalyzed precipitation of the enzyme product onto the sensor measured by non-Faradaic impedance. This sensor configuration had the best detection limit (DL) of 5 pg mL−1 and the best linear range of over 5 orders of magnitude (5 pg mL−1 to 1 µg mL−1) for IgY antibody in undiluted calf serum. This DL was 100-fold lower than label-free impedimetric immunosensors (0.5 ng mL−1), and 60-fold lower than when using HRP-Ab2 in amperometric immunosensors (0.3 ng mL−1).
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影响因子：
7.4
作者：
Patolsky, F;Zayats, M;Willner, I
通讯作者：
Willner, I
影响因子：
17.1
作者：
Mani, Vigneshwaran;Chikkaveeraiah, Bhaskara V.;Patel, Vyomesh;Gutkind, J. Silvio;Rusling, James F.
通讯作者：
Rusling, James F.
影响因子：
5.4
作者：
Chikkaveeraiah BV;Liu H;Mani V;Papadimitrakopoulos F;Rusling JF
通讯作者：
Rusling JF
影响因子：
14.2
作者：
Beyer, K;Ellman-Grunther, L;Sampson, HA
通讯作者：
Sampson, HA
影响因子：
14.2
作者：
BURKS, AW;WILLIAMS, LW;HELM, RM
通讯作者：
HELM, RM