Jianpi Qingchang decoction regulates intestinal motility of dextran sulfate sodium-induced colitis through reducing autophagy of interstitial cells of Cajal.
Jianpi Qingchang decoction regulates intestinal motility of dextran sulfate sodium-induced colitis through reducing autophagy of interstitial cells of Cajal.
复制标题
健脾清肠汤通过减少Cajal间质细胞自噬调节右旋糖酐硫酸钠诱导的结肠炎肠道动力
DOI：
10.3748/wjg.v23.i26.4724
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发表时间：
2017-07-14
影响因子：
4.3
通讯作者：
Tang ZP
中科院分区：
文献类型：
--
作者：
Dai YC;Zheng L;Zhang YL;Chen X;Chen DL;Wang LJ;Tang ZP
目的探讨健脾清肠汤(JQD)对右旋糖酐硫酸钠(DSS)所致小鼠结肠炎肠道蠕动的调节作用。方法C57BL/6小鼠随机分为4组：对照组、DSS组、JQD组、5-氨基水杨酸组。除对照组外，其余各组均采用含5%DSS的蒸馏水诱发结肠炎。造模后7天，给小鼠灌胃相应药物。第15天处死小鼠。观察疾病活动指数、肉眼及组织病理学病变以及Cajal结肠间质细胞(ICC)超微结构。肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-1β、IL-10和干扰素γ（IFN-γ）的水平，核因子κB（NF-κB）p65、c-kit、微管相关蛋白1轻链3（LC3-II）和Beclin-l mRNA的表达以及结肠平滑肌张力 评估。结果 接受 DSS 治疗的小鼠出现急性炎症。与对照组比较，DSS组IL-1β、TNF-α、IL-10、IFN-γ水平，LC3-II、Beclin-1、NF-κB p65 mRNA表达量及收缩频率升高（P < 0.05），c-kit mRNA表达量及结肠平滑肌收缩幅度降低（P < 0.05）。与DSS组比较，JQD组IL-10、IFN-γ水平、c-kit mRNA表达量、结肠平滑肌收缩幅度升高（P < 0.05），TNF-α、IL-1β水平，LC3-II、Beclin-1、NF-κB p65 mRNA表达量、收缩频率降低（P < 0.05）。结论金芪汤可通过抑制肠道炎症级联反应、减少ICC自噬、调节ICC/平滑肌细胞网络路径来调节DSS诱导的结肠炎小鼠肠道蠕动。
AIM To investigate the underlying effect of Jianpi Qingchang decoction (JQD) regulating intestinal motility of dextran sulfate sodium (DSS)-induced colitis in mice. METHODS C57BL/6 mice were randomly divided into four groups: the control group, the DSS group, the JQD group, and the 5-aminosalicylic acid group. Except for the control group, colitis was induced in other groups by giving distilled water containing 5% DSS. Seven days after modeling, the mice were administered corresponding drugs intragastrically. The mice were sacrificed on the 15th day. The disease activity index, macroscopic and histopathologic lesions, and ultrastructure of colon interstitial cells of Cajal (ICC) were observed. The levels of tumor necrosis factor-alpha (TNF-α), interleukin (IL)-1β, IL-10 and interferon gamma (IFN-γ), the expression of nuclear factor-kappa B (NF-κB) p65, c-kit, microtubule-associated protein 1 light chain 3 (LC3-II) and Beclin-l mRNA, and the colonic smooth muscle tension were assessed. RESULTS Acute inflammation occurred in the mice administered DSS. Compared with the control group, the levels of IL-1β, TNF-α, IL-10 and IFN-γ, the expression of LC3-II, Beclin-1 and NF-κB p65 mRNA, and the contractile frequency increased (P < 0.05), the expression of c-kit mRNA and the colonic smooth muscle contractile amplitude decreased in the DSS group (P < 0.05). Compared with the DSS group, the levels of IL-10 and IFN-γ, the expression of c-kit mRNA, and the colonic smooth muscle contractile amplitude increased (P < 0.05), the levels of TNF-α and IL-1β, the expression of LC3-II, Beclin-1 and NF-κB p65 mRNA, and the contractile frequency decreased in the JQD group (P < 0.05). CONCLUSION JQD can regulate the intestinal motility of DSS-induced colitis in mice through suppressing intestinal inflammatory cascade reaction, reducing autophagy of ICC, and regulating the network path of ICC/smooth muscle cells.
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影响因子：
5.1
作者：
Alkahtani, Reem;Mahavadi, Sunila;Al-Shboul, Othman;Alsharari, Shakir;Grider, John R.;Murthy, Karnam S.
通讯作者：
Murthy, Karnam S.
影响因子：
--
作者：
Ledesma-Soto Y;Callejas BE;Terrazas CA;Reyes JL;Espinoza-Jiménez A;González MI;León-Cabrera S;Morales R;Olguín JE;Saavedra R;Oghumu S;Satoskar AR;Terrazas LI
通讯作者：
Terrazas LI
影响因子：
2.3
作者：
Kinoshita, Kazuya;Horiguchi, Kazuhide;Ozaki, Hiroshi
通讯作者：
Ozaki, Hiroshi
影响因子：
4.3
作者：
Nam, Young-Sun;Kim, Nayoun;Cho, Seok-Goo
通讯作者：
Cho, Seok-Goo
影响因子：
4.9
作者：
Clapper, Margie L.;Gary, Monique A.;Cooper, Harry S.
通讯作者：
Cooper, Harry S.