Changes in the expression of smooth muscle contractile proteins in TNBS- and DSS-induced colitis in mice.
Changes in the expression of smooth muscle contractile proteins in TNBS- and DSS-induced colitis in mice.
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DOI：
10.1007/s10753-013-9669-0
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发表时间：
2013-12
期刊：
影响因子：
5.1
通讯作者：
Murthy, Karnam S.
中科院分区：
文献类型：
--
作者：
Alkahtani, Reem;Mahavadi, Sunila;Al-Shboul, Othman;Alsharari, Shakir;Grider, John R.;Murthy, Karnam S.
细丝相关蛋白如钙钙蛋白、钙钙素、原肌球蛋白和平滑蛋白被认为调节肌动蛋白-肌球蛋白相互作用，从而调节肌肉收缩。然而，炎症对细丝相关蛋白表达的影响尚不清楚。本研究的目的是确定TNBS和dss诱导的小鼠结肠炎结肠平滑肌中钙钙蛋白、钙调素、原肌球蛋白和平滑蛋白的表达变化。采用qRT-PCR和western blot检测h-caldesmon、h2-calponin、α-原肌球蛋白和smoothelin-A的表达。用扫描显微法测定分散肌细胞对乙酰胆碱的收缩反应。TNBS和dss诱导结肠炎小鼠结肠肌条中α-actin、h2-calponin、h-caldesmon、smooth - thelin和α-原肌球蛋白mRNA和蛋白表达量均显著高于对照组。与对照小鼠相比，从TNBS或dss治疗小鼠的炎症区域分离的肌肉细胞对乙酰胆碱的收缩反应明显减少。我们的研究结果表明，抑制肌动蛋白与肌球蛋白相互作用的薄丝相关收缩蛋白表达的增加可能有助于减少炎症时平滑肌收缩。
Thin filament associated proteins such as calponin, caldesmon, tropomyosin and smoothelin are thought to regulate acto-myosin interaction and thus, muscle contraction. However, the effect of inflammation on the expression of thin filament-associated proteins is not known. The aim of the present study is to determine the changes in the expression of calponin, caldesmon, tropomyosin, and smoothelin in colonic smooth muscle from TNBS- and DSS-induced colitis in mice. Expression of h-caldesmon, h2-calponin, α-tropomyosin and smoothelin-A was measured by qRT-PCR, and western blot. Contraction in response to acetylcholine in dispersed muscle cells was measured by scanning micrometry. mRNA and protein expression of α-actin, h2-calponin, h-caldesmon, smoothelin and α-tropomyosin in colonic muscle strips from mice with TNBS- or DSS-induced colitis was significantly increased compared to control animals. Contraction in response to acetylcholine was significantly decreased in muscle cells isolated from inflamed regions of TNBS- or DSS-treated mice compared to control mice. Our results show that increase in the expression of thin-filament associated contractile proteins, which inhibit acto-myosin interaction, could contribute to decrease in smooth muscle contraction in inflammation.
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影响因子：
3.5
作者：
Akbarali HI;G Hawkins E;Ross GR;Kang M
通讯作者：
Kang M
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影响因子：
3.7
作者：
Bushell KN;Leeman SE;Gillespie E;Gower AC;Reed KL;Stucchi AF;Becker JM;Amar S
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影响因子：
3.5
作者：
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Grider, JR
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10.1152/ajpgi.00545.2006
发表时间：
2007-07-01
影响因子：
4.5
作者：
Akiho, Hirotada;Khan, Waliul I.;Collins, Stephen M.
通讯作者：
Collins, Stephen M.
影响因子：
3.1
作者：
Gaudio, E;Taddei, G;Caprilli, R
通讯作者：
Caprilli, R