A nuclear-localized cysteine desulfhydrase plays a role in fruit ripening in tomato.
A nuclear-localized cysteine desulfhydrase plays a role in fruit ripening in tomato.
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核定位的半胱氨酸脱硫酶在番茄果实成熟中发挥作用
DOI：
10.1038/s41438-020-00439-1
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发表时间：
2020-12-14
影响因子：
8.7
通讯作者：
Zhang H
中科院分区：
文献类型：
--
作者：
Hu KD;Zhang XY;Yao GF;Rong YL;Ding C;Tang J;Yang F;Huang ZQ;Xu ZM;Chen XY;Li YH;Hu LY;Zhang H
硫化氢（H2S）是一种气体信号分子，在植物发育过程中起着多种作用。然而，内源H2S是否在番茄果实成熟过程中起作用尚不清楚。在这项研究中，我们发现产生h2s的酶l-半胱氨酸脱氢酶SlLCD1定位于细胞核。通过构建SlLCD1的突变形式，我们发现SlLCD1的氨基酸残基K24是决定核定位的关键氨基酸。与对照相比，TRV-SlLCD1对SlLCD1的沉默促进了果实成熟，减少了H2S的产生。通过CRISPR/Cas9修饰获得的SlLCD1基因编辑突变体表现出略微矮化的表型和加速的果实成熟。该突变体还显示出半胱氨酸含量增加，产生的H2S减少，表明SlLCD1在H2S产生中的作用。SlLCD1突变体的叶绿素降解和类胡萝卜素积累增强。在lcd1突变体中，其他在叶绿素降解、类胡萝卜素生物合成、细胞壁降解、乙烯生物合成和乙烯信号通路中发挥作用的成熟相关基因在转录水平上得到增强。对外源H2S处理的未成熟番茄果实进行了总RNA测序，转录组分析显示成熟相关基因表达受到抑制。基于SlLCD1基因编辑突变体和外源H2S应用的结果，我们提出核定位的半胱氨酸脱氢酶SlLCD1是内源H2S生成所必需的，并参与番茄果实成熟的调控。
Hydrogen sulfide (H2S) is a gaseous signaling molecule that plays multiple roles in plant development. However, whether endogenous H2S plays a role in fruit ripening in tomato is still unknown. In this study, we show that the H2S-producing enzyme l-cysteine desulfhydrase SlLCD1 localizes to the nucleus. By constructing mutated forms of SlLCD1, we show that the amino acid residue K24 of SlLCD1 is the key amino acid that determines nuclear localization. Silencing of SlLCD1 by TRV-SlLCD1 accelerated fruit ripening and reduced H2S production compared with the control. A SlLCD1 gene-edited mutant obtained through CRISPR/Cas9 modification displayed a slightly dwarfed phenotype and accelerated fruit ripening. This mutant also showed increased cysteine content and produced less H2S, suggesting a role of SlLCD1 in H2S generation. Chlorophyll degradation and carotenoid accumulation were enhanced in the SlLCD1 mutant. Other ripening-related genes that play roles in chlorophyll degradation, carotenoid biosynthesis, cell wall degradation, ethylene biosynthesis, and the ethylene signaling pathway were enhanced at the transcriptional level in the lcd1 mutant. Total RNA was sequenced from unripe tomato fruit treated with exogenous H2S, and transcriptome analysis showed that ripening-related gene expression was suppressed. Based on the results for a SlLCD1 gene-edited mutant and exogenous H2S application, we propose that the nuclear-localized cysteine desulfhydrase SlLCD1 is required for endogenous H2S generation and participates in the regulation of tomato fruit ripening.
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