Control of functional mRNA stability in bacteria: multiple mechanisms of nucleolytic and non‐nucleolytic inactivation
Control of functional mRNA stability in bacteria: multiple mechanisms of nucleolytic and non‐nucleolytic inactivation
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细菌功能性 mRNA 稳定性的控制：溶核和非溶核失活的多种机制
作者：
C. Petersen
细菌中的信使RNA可以通过几种独立作用于不同靶位点的平行机制失活。对于任何种类的mRNA，总失活速率由不同机制的贡献之和决定。转录本可以通过核酸内切攻击直接失活，也可以通过进行性核酸裂解降解失活，这可能在3′-5′方向进行，也可能在5′-3 ′方向进行。此外，许多mRNA的功能寿命可能由非溶核的过程决定，例如翻译阻遏物的结合或阻止翻译起始的二级结构的形成。这些非溶核过程也可能决定化学稳定性，因为化学降解通常与功能失活密切相关。不同的机制在散装细胞mRNA的失活的相对重要性，以及稳定的mRNA的工程的一般前景进行了讨论。
Messenger RNA in bacteria may be inactivated by several parallel mechanisms acting independently on different target sites. For any species of mRNA the overall rate of inactivation is determined by the sum of the contributions from the different mechanisms. Transcripts may be inactivated directly by endonucleolytic attack or by processive nucleolytic degradation, which may proceed in the 3′–5′ direction and probably also in the 5′‐3′ direction. Moreover, the functional lifetime of many mRNAs may be determined by processes that are not nucleolytic, such as the binding of translational repressors or the formation of secondary structures which prevent initiation of translation. These non‐nucleolytic processes may also determine the chemical stability as chemical degradation frequently appears to be closely coupled to functional inactivation. The relative importance of the different mechanisms in the inactivation of bulk cellular mRNA, as well as the general prospects for engineering of stable mRNAs are discussed.
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DOI：
--
发表时间：
1989
期刊：
The New biologist
影响因子：
--
作者：
Ruckman,J;Parma,D;Tuerk,C;Hall,DH;Gold,L
通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
Green,PJ;Inouye,M
通讯作者：
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DOI：
10.1073/pnas.83.1.120
发表时间：
1986-01-01
影响因子：
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通讯作者：
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