Detection of astrocytic tau pathology facilitates recognition of chronic traumatic encephalopathy neuropathologic change.
Detection of astrocytic tau pathology facilitates recognition of chronic traumatic encephalopathy neuropathologic change.
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星形细胞tau病理检测有助于识别慢性外伤性脑病的神经病理改变。
DOI：
10.1186/s40478-022-01353-4
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发表时间：
2022-04-11
影响因子：
7.1
通讯作者：
CONNECT-TBI Investigators
中科院分区：
文献类型：
--
作者：
Ameen-Ali KE;Bretzin A;Lee EB;Folkerth R;Hazrati LN;Iacono D;Keene CD;Kofler J;Kovacs GG;Nolan A;Perl DP;Priemer DS;Smith DH;Wiebe DJ;Stewart W;CONNECT-TBI Investigators
创伤性脑损伤（TBI）与一系列神经退行性病变的发展相关，包括慢性创伤性脑病（CTE）。目前的共识诊断标准将CTE神经病理学变化（CTE-NC）的特异性皮质病变定义为神经元中过度磷酸化tau的斑片状沉积，在刺状星形胶质细胞中有或没有胶质tau，通常朝向脑沟深处并聚集在小血管周围。然而，尽管纳入共识诊断标准，单个细胞组分对CTE-NC鉴定的贡献尚未得到正式评估。为了解决这一问题，从格拉斯哥TBI档案中，从来自接触性体育运动员的连续脑捐赠中选择皮质组织块，其中已知存在CTE-NC（n = 12）或阿尔茨海默病神经病理学变化（n = 4）。从这些组织块中，对相邻组织切片进行tau抗体染色，选择tau抗体以揭示单独的神经元病理学（3R tau; GT-38）或混合的神经元和星形胶质细胞病理学（4 R tau; PHF-1）。然后将这些染色切片随机化，并由一组专家神经病理学家独立评估，这些专家对患者临床病史和应用于每个切片的一抗不知情，要求他们记录是否存在CTE-NC。结果表明，在4 R tau或PHF-1染色的切片中，CTE-NC的一致识别是高的。相比之下，在3R tau或GT-38染色的切片中CTE-NC的识别很差;在前者中并不比机会好。我们的观察结果表明，神经元和星形胶质细胞tau病理的存在有利于CTE-NC的检测，当单独的神经元tau病理可见时，其检测不太一致。因此，CTE-NC的检测可能需要神经胶质和神经元病理的组合。
Traumatic brain injury (TBI) is associated with the development of a range of neurodegenerative pathologies, including chronic traumatic encephalopathy (CTE). Current consensus diagnostic criteria define the pathognomonic cortical lesion of CTE neuropathologic change (CTE-NC) as a patchy deposition of hyperphosphorylated tau in neurons, with or without glial tau in thorn-shaped astrocytes, typically towards the depths of sulci and clustered around small blood vessels. Nevertheless, although incorporated into consensus diagnostic criteria, the contribution of the individual cellular components to identification of CTE-NC has not been formally evaluated. To address this, from the Glasgow TBI Archive, cortical tissue blocks were selected from consecutive brain donations from contact sports athletes in which there was known to be either CTE-NC (n = 12) or Alzheimer’s disease neuropathologic change (n = 4). From these tissue blocks, adjacent tissue sections were stained for tau antibodies selected to reveal either solely neuronal pathology (3R tau; GT-38) or mixed neuronal and astroglial pathologies (4R tau; PHF-1). These stained sections were then randomised and independently assessed by a panel of expert neuropathologists, blind to patient clinical history and primary antibody applied to each section, who were asked to record whether CTE-NC was present. Results demonstrate that, in sections stained for either 4R tau or PHF-1, consensus recognition of CTE-NC was high. In contrast, recognition of CTE-NC in sections stained for 3R tau or GT-38 was poor; in the former no better than chance. Our observations demonstrate that the presence of both neuronal and astroglial tau pathologies facilitates detection of CTE-NC, with its detection less consistent when neuronal tau pathology alone is visible. The combination of both glial and neuronal pathologies, therefore, may be required for detection of CTE-NC.
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影响因子：
7.1
作者：
Kovacs GG;Xie SX;Robinson JL;Lee EB;Smith DH;Schuck T;Lee VM;Trojanowski JQ
通讯作者：
Trojanowski JQ
影响因子：
3.2
作者：
Bieniek KF;Cairns NJ;Crary JF;Dickson DW;Folkerth RD;Keene CD;Litvan I;Perl DP;Stein TD;Vonsattel JP;Stewart W;Dams-O'Connor K;Gordon WA;Tripodis Y;Alvarez VE;Mez J;Alosco ML;McKee AC;TBI/CTE Research Group
通讯作者：
TBI/CTE Research Group
影响因子：
3.2
作者：
Doherty, Colin P.;O'Keefe, Eoin;Campbell, Matthew
通讯作者：
Campbell, Matthew
影响因子：
12.7
作者：
Kovacs GG;Ferrer I;Grinberg LT;Alafuzoff I;Attems J;Budka H;Cairns NJ;Crary JF;Duyckaerts C;Ghetti B;Halliday GM;Ironside JW;Love S;Mackenzie IR;Munoz DG;Murray ME;Nelson PT;Takahashi H;Trojanowski JQ;Ansorge O;Arzberger T;Baborie A;Beach TG;Bieniek KF;Bigio EH;Bodi I;Dugger BN;Feany M;Gelpi E;Gentleman SM;Giaccone G;Hatanpaa KJ;Heale R;Hof PR;Hofer M;Hortobágyi T;Jellinger K;Jicha GA;Ince P;Kofler J;Kövari E;Kril JJ;Mann DM;Matej R;McKee AC;McLean C;Milenkovic I;Montine TJ;Murayama S;Lee EB;Rahimi J;Rodriguez RD;Rozemüller A;Schneider JA;Schultz C;Seeley W;Seilhean D;Smith C;Tagliavini F;Takao M;Thal DR;Toledo JB;Tolnay M;Troncoso JC;Vinters HV;Weis S;Wharton SB;White CL 3rd;Wisniewski T;Woulfe JM;Yamada M;Dickson DW
通讯作者：
Dickson DW
影响因子：
14.5
作者：
Arena, John D.;Smith, Douglas H.;Johnson, Victoria E.
通讯作者：
Johnson, Victoria E.