Culture and hybridization experiments on an ulva clade including the Qingdao strain blooming in the yellow sea.
Culture and hybridization experiments on an ulva clade including the Qingdao strain blooming in the yellow sea.
复制标题
DOI：
10.1371/journal.pone.0019371
复制
发表时间：
2011-05-05
期刊：
影响因子：
3.7
通讯作者：
Shimada S
中科院分区：
文献类型：
--
作者：
Hiraoka M;Ichihara K;Zhu W;Ma J;Shimada S
2008年夏天，就在北京奥运会之前，一场巨大的绿色潮覆盖了中国黄海的青岛海岸。基于核编码rDNA内转录间隔区（ITS）序列的分子分析，青岛绿色潮的优势菌株被划分为一个进化支，目前被认为是一个单一的种。另一方面，我们详细的系统发育分析的分支，使用更高分辨率的DNA标记，表明两个遗传上独立的实体可以包括在分支。然而，石莼分支内的物种形成尚未被研究。我们研究了一个复杂的物种形成的分支内，包括青岛株，通过文化，杂交和系统发育分析相结合的研究。通过系统发育分析，将这两个实体简单地区分为U。linza和U.通过培养的菌体中分支的存在或缺失而在形态上增殖。在每个分类群中都发现了有性株和几个无性株，有性株之间的杂交也支持了部分配子不亲和性的分离。有性繁殖的青岛品系与美国种间杂交。prolifera没有任何生殖边界，但对U. linza由配子不亲和性引起。研究结果表明，美国在这一领域的研究具有重要意义。prolifera组包括两种类型的性菌株，可通过与U.林莎。石莼进化枝内的物种鉴定需要高分辨率的DNA标记和/或杂交实验，并且仅依赖ITS标记是不可能的。
In the summer of 2008, immediately prior to the Beijing Olympics, a massive green tide of the genus Ulva covered the Qingdao coast of the Yellow Sea in China. Based on molecular analyses using the nuclear encoded rDNA internal transcribed spacer (ITS) region, the Qingdao strains dominating the green tide were reported to be included in a single phylogenetic clade, currently regarded as a single species. On the other hand, our detailed phylogenetic analyses of the clade, using a higher resolution DNA marker, suggested that two genetically separate entities could be included within the clade. However, speciation within the Ulva clade has not yet been examined. We examined the occurrence of an intricate speciation within the clade, including the Qingdao strains, via combined studies of culture, hybridization and phylogenetic analysis. The two entities separated by our phylogenetic analyses of the clade were simply distinguished as U. linza and U. prolifera morphologically by the absence or presence of branches in cultured thalli. The inclusion of sexual strains and several asexual strains were found in each taxon. Hybridizations among the sexual strains also supported the separation by a partial gamete incompatibility. The sexually reproducing Qingdao strains crossed with U. prolifera without any reproductive boundary, but a complete reproductive isolation to U. linza occurred by gamete incompatibility. The results demonstrate that the U. prolifera group includes two types of sexual strains distinguishable by crossing affinity to U. linza. Species identification within the Ulva clade requires high resolution DNA markers and/or hybridization experiments and is not possible by reliance on the ITS markers alone.
登录
查看更多内容
影响因子：
4.8
作者：
Nelson, Timothy A.;Haberlin, Karalon;Fredrickson, Kerri
通讯作者：
Fredrickson, Kerri
影响因子：
4.9
作者：
Leskinen, E;Alström-Rapaport, C;Pamilo, P
通讯作者：
Pamilo, P
影响因子：
1.5
作者：
Leliaert, Frederik;Zhang, Xiaowen;De Clerck, Olivier
通讯作者：
De Clerck, Olivier
影响因子：
2.2
作者：
Nelson, TA;Nelson, AV;Tjoelker, M
通讯作者：
Tjoelker, M
影响因子：
4.5
作者：
Mueller, Tobias;Philippi, Nicole;Wolf, Matthias
通讯作者：
Wolf, Matthias