Proteomic and network analysis characterize stage-specific metabolism in Trypanosoma cruzi.
Proteomic and network analysis characterize stage-specific metabolism in Trypanosoma cruzi.
复制标题
DOI：
10.1186/1752-0509-3-52
复制
发表时间：
2009-05-16
影响因子：
--
通讯作者：
Buck GA
中科院分区：
文献类型：
--
作者：
Roberts SB;Robichaux JL;Chavali AK;Manque PA;Lee V;Lara AM;Papin JA;Buck GA
Trypanosoma cruzi is a Kinetoplastid parasite of humans and is the cause of Chagas disease, a potentially lethal condition affecting the cardiovascular, gastrointestinal, and nervous systems of the human host. Constraint-based modeling has emerged in the last decade as a useful approach to integrating genomic and other high-throughput data sets with more traditional, experimental data acquired through decades of research and published in the literature. We present a validated, constraint-based model of the core metabolism of Trypanosoma cruzi strain CL Brener. The model includes four compartments (extracellular space, cytosol, mitochondrion, glycosome), 51 transport reactions, and 93 metabolic reactions covering carbohydrate, amino acid, and energy metabolism. In addition, we make use of several replicate high-throughput proteomic data sets to specifically examine metabolism of the morphological form of T. cruzi in the insect gut (epimastigote stage). This work demonstrates the utility of constraint-based models for integrating various sources of data (e.g., genomics, primary biochemical literature, proteomics) to generate testable hypotheses. This model represents an approach for the systematic study of T. cruzi metabolism under a wide range of conditions and perturbations, and should eventually aid in the identification of urgently needed novel chemotherapeutic targets.
登录
查看更多内容
影响因子：
1.5
作者：
CAZZULO, JJ;DECAZZULO, BMF;CANNATA, JJB
通讯作者：
CANNATA, JJB
影响因子：
2.4
作者：
CROSS, GAM;KLEIN, RA;LINSTEAD, DJ
通讯作者：
LINSTEAD, DJ
扁桃体周围脓肿临床结果与微生物学发现之间的关联——一项观察性研究
DOI：
10.1007/s10096-025-05156-y
发表时间：
2025-05-14
影响因子：
3.000
作者：
Elin Lindberg;Ann Hermansson;David Nygren;Marie Gisselsson-Solén
通讯作者：
Marie Gisselsson-Solén
DOI：
10.1073/pnas.96.8.4273
发表时间：
1999-04-13
影响因子：
11.1
作者：
Aronov, AM;Suresh, S;Gelb, MH
通讯作者：
Gelb, MH
影响因子：
4.1
作者：
Fast, B;Kremp, K;Steverding, D
通讯作者：
Steverding, D