Structure Determination of Microtubules and Pili: Past, Present, and Future Directions.
Structure Determination of Microtubules and Pili: Past, Present, and Future Directions.
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DOI：
10.3389/fmolb.2021.830304
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发表时间：
2021
影响因子：
5
通讯作者：
Atherton J
中科院分区：
文献类型：
--
作者：
Garnett JA;Atherton J
历史上，形成高度聚合和丝状组件的蛋白质一直很难使用高分辨率结构技术进行研究。这是由于多种因素造成的，包括结构异质性、大分子量和可用产量。然而，在过去的十年中，我们现在看到了向原子分辨率洞察、更复杂的异质样品的研究以及多亚基复合物的原位/离体检查的重大转变。尽管得到了固态核磁共振波谱 (ssNMR) 和计算方法发展的支持，但这主要归功于低温电子显微镜 (cryo-EM) 的进步。对真核微管和细菌菌毛的研究就是很好的例子，在这篇综述中，我们将概述实现这一转变的技术创新，并重点介绍这两个系统所取得的进步。展望未来，我们还将描述仍然难以研究以及需要进一步技术突破的系统。
Historically proteins that form highly polymeric and filamentous assemblies have been notoriously difficult to study using high resolution structural techniques. This has been due to several factors that include structural heterogeneity, their large molecular mass, and available yields. However, over the past decade we are now seeing a major shift towards atomic resolution insight and the study of more complex heterogenous samples and in situ/ex vivo examination of multi-subunit complexes. Although supported by developments in solid state nuclear magnetic resonance spectroscopy (ssNMR) and computational approaches, this has primarily been due to advances in cryogenic electron microscopy (cryo-EM). The study of eukaryotic microtubules and bacterial pili are good examples, and in this review, we will give an overview of the technical innovations that have enabled this transition and highlight the advancements that have been made for these two systems. Looking to the future we will also describe systems that remain difficult to study and where further technical breakthroughs are required.
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DOI：
10.1126/science.aad2001
发表时间：
2016-03-11
期刊：
Science (New York, N.Y.)
影响因子：
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作者：
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DOI：
10.1073/pnas.2001546117
发表时间：
2020-07-21
影响因子：
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