Development of a filter device for the prevention of aquatic bacterial disease using a single-chain variable fragment (scFv)-conjugated affinity silk.
Development of a filter device for the prevention of aquatic bacterial disease using a single-chain variable fragment (scFv)-conjugated affinity silk.
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DOI：
10.1038/s41598-022-13408-6
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发表时间：
2022-06-08
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
--
作者：
传染病是观赏鱼或食用鱼养殖业中最严重的问题之一。本研究试图利用“亲和丝”开发一种预防由杀鲑鱼气单胞菌（As）引起的水生细菌性疾病--溃疡病的新装置。亲和丝是融合到单链可变片段（scFv）的含有丝蛋白的丝心蛋白L链（FibL）。它可以很容易地加工成不同的形式，如纤维，凝胶，海绵或薄膜。成功地产生了可以表达含有与来自抗As的单克隆抗体（MAb）的scFv融合的FibL的cDNA构建体的转基因家蚕。酶联免疫吸附试验用于检测通过采用96孔板与scFv-缀合的亲和丝包被。吸附柱中涂有亲和丝的玻璃棉能有效地捕集As。此外，配备有亲和丝涂层羊毛的气升式水过滤器可以大大降低水中的As浓度，估计有足够的能力捕获致死剂量的As。这些研究结果表明，“亲和丝滤器”是一种潜在的预防水生动物疾病的装置。
Infectious disease is one of the most serious problems in the aquaculture industry for ornamental or edible fish. This study attempted to develop a new device for preventing an aquatic bacterial disease, ulcer disease, caused by Aeromonas salmonicida (As), using “affinity silk”. Affinity silk is a silk protein-containing fibroin L-chain (FibL) fused to the single-chain variable fragment (scFv). It can be easily processed into different formats such as fibers, gels, sponges, or films. A transgenic silkworm that could express a cDNA construct containing FibL fused to an scFv derived from a monoclonal antibody (MAb) against As was successfully generated. An enzyme-linked immunosorbent assay was used to detect As by employing 96-well plates coated with scFv-conjugated affinity silk. As could be captured efficiently by glass wool coated with affinity silk in the column. Furthermore, the air-lift water filter equipped with the affinity silk-coated wool could considerably reduce the concentration of As in water and was estimated to have sufficient ability to trap a lethal dose of As. These findings show that the “affinity silk filter” is a potential device for the prophylaxis of aquatic animal diseases.
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DOI：
10.1016/j.cvex.2020.01.004
发表时间：
2020-05-01
期刊：
Veterinary Clinics of North America Exotic Animal Practice
影响因子：
--
作者：
McDermott, Colin;Palmeiro, Brian
通讯作者：
Palmeiro, Brian
DOI：
10.20506/rst.27.1.1788
发表时间：
2008-04-01
影响因子：
0.6
作者：
Subasinghe, R. P.;Bondad-Reantaso, M. G.
通讯作者：
Bondad-Reantaso, M. G.
影响因子：
4.6
作者：
Sato M;Kojima K;Sakuma C;Murakami M;Tamada Y;Kitani H
通讯作者：
Kitani H
影响因子：
1.6
作者：
Ikura, K;Kodama, S;Hara, S
通讯作者：
Hara, S
DOI：
10.1016/j.cvex.2006.05.027
发表时间：
2006-09-01
期刊：
The veterinary clinics of North America. Exotic animal practice
影响因子：
--
作者：
Hunt, Craig J-G
通讯作者：
Hunt, Craig J-G