Aqueous cationic, anionic and non-ionic multi-walled carbon nanotubes, functionalised with minimal framework damage, for biomedical application.
Aqueous cationic, anionic and non-ionic multi-walled carbon nanotubes, functionalised with minimal framework damage, for biomedical application.
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DOI：
10.1016/j.biomaterials.2014.02.002
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发表时间：
2014-06
期刊：
影响因子：
14
通讯作者：
Shaffer, Milo S. P.
中科院分区：
文献类型：
--
作者：
Chen, Shu;Hu, Sheng;Smith, Elizabeth F.;Ruenraroengsak, Pakatip;Thorley, Andrew J.;Menzel, Robert;Goode, Angela E.;Ryan, Mary P.;Tetley, Teresa D.;Porter, Alexandra E.;Shaffer, Milo S. P.
关键词：
热化学接枝方法的使用提供了一种多功能的手段来官能化合成的块状多壁碳纳米管（MWNT），而不改变其固有结构。相关性质的保留对于广泛的商业应用（包括药物输送和医疗目的）来说是理想的;它也与内在毒理学研究有关。已经制备了一系列直径约为12 nm的与水相容的MWNT，以提供主要由阴离子、阳离子或非离子基团稳定的结构等效样品。通过改变接枝试剂和随后的后官能化修饰来控制MWNTs的表面电荷。通过热分析（TGA）确定接枝度。高分辨率透射电子显微镜（HRTEM）和拉曼测量证实，MWNTs的结构框架是不受热化学处理，相比之下，传统的酸氧化的控制，这是严重损坏。表面改性的有效性通过在水和细胞培养基中显著改善的溶解度和稳定性来证明，并通过ζ-电位分析进一步定量。通过3-（4，5-二甲基噻唑-2-基）-5-（3-羧基甲氧基苯基）-2-（4-磺基苯基）-2H-四唑（MTS）和乳酸脱氢酶释放（LDH）测定证实，在暴露24小时后，移植的MWNTs对人永生化肺泡上皮1型样细胞（TT 1）表现出相对较低的生物活性。TT 1细胞暴露于MWNTs抑制了炎症介质白细胞介素6（IL-6）和白细胞介素8（IL-8）的释放。TEM细胞摄取的研究表明，有效的细胞进入的MWNTs到TT 1细胞，通过一系列的机制。阳离子多壁碳纳米管表现出更实质性的相互作用与TT 1细胞膜比阴离子多壁碳纳米管，表现出表面电荷对细胞摄取的影响。
The use of a thermochemical grafting approach provides a versatile means to functionalise as-synthesised, bulk multi-walled carbon nanotubes (MWNTs) without altering their inherent structure. The associated retention of properties is desirable for a wide range of commercial applications, including for drug delivery and medical purposes; it is also pertinent to studies of intrinsic toxicology. A systematic series of water-compatible MWNTs, with diameter around 12 nm have been prepared, to provide structurally-equivalent samples predominantly stabilised by anionic, cationic, or non-ionic groups. The surface charge of MWNTs was controlled by varying the grafting reagents and subsequent post-functionalisation modifications. The degree of grafting was established by thermal analysis (TGA). High resolution transmission electron microscope (HRTEM) and Raman measurements confirmed that the structural framework of the MWNTs was unaffected by the thermochemical treatment, in contrast to a conventional acid-oxidised control which was severely damaged. The effectiveness of the surface modification was demonstrated by significantly improved solubility and stability in both water and cell culture medium, and further quantified by zeta-potential analysis. The grafted MWNTs exhibited relatively low bioreactivity on human immortal alveolar epithelial type 1-like cells (TT1) following 24h exposure as demonstrated by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) and lactate dehydrogenase release (LDH) assays. The exposure of TT1 cells to MWNTs suppressed the release of the inflammatory mediators, interleukin 6 (IL-6) and interleukin 8 (IL-8). TEM cell uptake studies indicated efficient cellular entry of MWNTs into TT1 cells, via a range of mechanisms. Cationic MWNTs showed a more substantial interaction with TT1 cell membranes than anionic MWNTs, demonstrating a surface charge effect on cell uptake.
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影响因子：
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