Secreted Glycosyltransferase RsIA_GT of Rhizoctonia solani AG-1 IA Inhibits Defense Responses in Nicotiana benthamiana.
Secreted Glycosyltransferase RsIA_GT of Rhizoctonia solani AG-1 IA Inhibits Defense Responses in Nicotiana benthamiana.
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根源部的分泌的糖基转移酶RSIA_GT Solani ag-1 Ia抑制了Nicotiana Benthamiana的防御反应。
DOI：
10.3390/pathogens11091026
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发表时间：
2022-09-09
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影响因子：
--
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中科院分区：
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立枯丝核菌(Rhizoctonia solani Khün)吻合群AG-1 IA寄主范围广，对农作物生产构成威胁。植物病原真菌分泌的各种糖基转移酶在致病性中发挥重要作用。此前，我们鉴定了糖基转移酶 RsIA_GT (AG11A_09161) 作为立枯丝核菌 AG-1 IA 的分泌蛋白编码基因，其表达水平在水稻感染期间增加。在本研究中，我们进一步表征了 RsIA_GT 的毒力功能。它不仅在担子菌门（包括立枯丝核菌的多个吻合群）中保守，而且在其他主要真菌分类类别中也保守。 RsIA_GT 拥有用于蛋白质分泌的信号肽 (SP)，其功能已通过酵母和本塞姆氏烟草得到证实。当应用于穿孔叶片时，RsIA_GT 的 SP 截短形式 (RsIA_GT(ΔS)) 在水稻叶片中大肠杆菌诱导的病灶样表型中表达。然而，农杆菌介导的全长 RsIA_GT 和 RsIA_GT(ΔS) 的瞬时表达不会诱导本塞姆氏烟草叶子中的细胞死亡。相反，在本塞姆氏烟草中，只有 RsIA_GT(ΔS) 抑制了两种参考细胞死亡因子 BAX 和 INF1 诱导的细胞死亡。 RsIA_GT(ΔS)R154A D168A D170A 是糖基转移酶催化结构域的突变体 RsIA_GT(ΔS)，仍然抑制 BAX 或 INF1 诱导的细胞死亡，表明 RsIA_GT(ΔS) 的细胞死亡抑制活性与其酶活性无关。 RsIA_GT(ΔS) 还抑制 H2O2 产生和胼胝质沉积，并对与 BAX 和 INF1 表达相关的防御基因的诱导产生影响。本塞姆氏烟草中RsIA_GT(ΔS)的瞬时表达增强了立枯丝核菌AG-1 IA引起的病斑面积。立枯丝核菌 AG-1 IA 分泌的糖基转移酶 RsIA_GT 可能在宿主细胞内外的毒力中具有双重作用。
Anastomosis group AG-1 IA of Rhizoctonia solani Khün has a wide host range and threatens crop production. Various glycosyltransferases secreted by phytopathogenic fungi play an essential role in pathogenicity. Previously, we identified a glycosyltransferase RsIA_GT (AG11A_09161) as a secreted protein-encoding gene of R. solani AG-1 IA, whose expression levels increased during infection in rice. In this study, we further characterized the virulence function of RsIA_GT. It is conserved not only in Basidiomycota, including multiple anastomosis groups of R. solani, but also in other primary fungal taxonomic categories. RsIA_GT possesses a signal peptide (SP) for protein secretion, and its functionality was proven using yeast and Nicotiana benthamiana. The SP-truncated form of RsIA_GT (RsIA_GT(ΔS)) expressed in Escherichia coli-induced lesion-like phenotype in rice leaves when applied to punched leaves. However, Agrobacterium-mediated transient expressions of both the full-length RsIA_GT and RsIA_GT(ΔS) did not induce cell death in N. benthamiana leaves. Instead, only RsIA_GT(ΔS) suppressed the cell death induced by two reference cell death factors BAX and INF1 in N. benthamiana. RsIA_GT(ΔS)R154A D168A D170A, a mutant RsIA_GT(ΔS) for the glycosyltransferase catalytic domain, still suppressed the BAX- or INF1-induced cell death, suggesting that the cell death suppression activity of RsIA_GT(ΔS) would be independent from its enzymatic activity. RsIA_GT(ΔS) also suppressed the H2O2 production and callose deposition and showed an effect on the induction of defense genes associated with the expression of BAX and INF1. The transient expression of RsIA_GT(ΔS) in N. benthamiana enhanced the lesion area caused by R. solani AG-1 IA. The secreted glycosyltransferase, RsIA_GT, of R. solani AG-1 IA is likely to have a dual role in virulence inside and outside of host cells.
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影响因子：
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