Annexin A7 suppresses lymph node metastasis of hepatocarcinoma cells in a mouse model.
Annexin A7 suppresses lymph node metastasis of hepatocarcinoma cells in a mouse model.
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膜联蛋白 A7 抑制小鼠模型中肝癌细胞的淋巴结转移。
DOI：
10.1186/1471-2407-13-522
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发表时间：
2013-11-04
期刊：
影响因子：
3.8
通讯作者：
Tang J
中科院分区：
文献类型：
--
作者：
Jin Y;Wang S;Chen W;Zhang J;Wang B;Guan H;Tang J
肝细胞癌（HCC）是我国癌症死亡的主要原因之一。本研究探讨了膜联蛋白A7（ANXA 7）对小鼠肝癌淋巴结转移的抑制作用。将分别使用膜联蛋白A7 cDNA和shRNA载体的膜联蛋白A7表达HCC细胞的稳定敲高和敲低注射到小鼠足垫中以在小鼠中建立原发性和转移性肿瘤。分别于接种肝癌细胞后第14、21、28天处死小鼠，进行原发性和继发性肿瘤的检查和Annexin A7表达的免疫组化检测。FANXA 7-对照组的淋巴结转移率为77%，FANXA 7-下组的淋巴结转移率为100%（p < 0.05）。相比之下，PANXA 7-up组的淋巴结转移率为0%，而PANXA 7-对照组的淋巴结转移率为36%（p < 0.05）。此外，免疫组化实验显示，在原发性和淋巴结转移肿瘤中，Annexin A7蛋白的亚细胞定位主要在胞浆中。此外，47 kDa和51 kDa亚型的膜联蛋白A7蛋白的表达在肿瘤进展过程中发生变化。该研究表明，Annexin A7表达能够抑制HCC淋巴结转移，而Annexin A7表达的敲低显著诱导HCC向局部淋巴结转移。
Hepatocellular carcinoma (HCC) is one of the leading causes of cancer death in China. This study investigated the effects of Annexin A7 (ANXA7) on the inhibition of HCC lymph node metastasis in a mouse model. The stable knockup and knockdown of Annexin A7-expressing HCC cells using Annexin A7 cDNA and shRNA vectors, respectively, were injected into a mouse footpad to establish primary and metastatic tumors in mice. On the 14th, 21st, and 28th days after HCC cells inoculation, the mice were sacrificed for inspection of primary and secondary tumors and immunohistochemistry of Annexin A7 expression. The lymph node metastasis rate of the FANXA7-control group was 77%, and the lymph node metastasis rate of the FANXA7-down group was 100% (p < 0.05). In contrast, the lymph node metastasis rate of the PANXA7-up group was 0% and that of the PANXA7-control group was 36% (p < 0.05). Furthermore, immunohistochemistry experiments revealed that the subcellular localization of Annexin A7 protein in both primary and lymph node-metastasized tumors was mainly in the cytosol. In addition, the expression of the 47 kDa and 51 kDa isoforms of Annexin A7 protein changed during tumor progression. This study indicated that Annexin A7 expression was able to inhibit HCC lymph node metastasis, whereas knockdown of Annexin A7 expression significantly induced HCC metastasis to local lymph nodes.
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DOI：
10.1016/j.biocel.2007.05.023
发表时间：
2007-01-01
影响因子：
4
作者：
Jia, Li;Wang, Shujing;Zhang, Jianing
通讯作者：
Zhang, Jianing
影响因子：
9.7
作者：
Leighton, X;Srikantan, V;Srivastava, M
通讯作者：
Srivastava, M
影响因子：
2.7
作者：
Clemen, CS;Hofmann, A;Noegel, AA
通讯作者：
Noegel, AA
DOI：
10.1073/pnas.2235927100
发表时间：
2003-11-25
影响因子：
11.1
作者：
Srivastava, M;Montagna, C;Pollard, HB
通讯作者：
Pollard, HB
DOI：
10.1016/0005-2736(87)90221-5
发表时间：
1987-10-02
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
NIR, S;STUTZIN, A;POLLARD, HB
通讯作者：
POLLARD, HB