Proteomic studies of the Arabidopsis TRAPP complexes reveal conserved organization and a novel plant-specific component with a role in plant development
Proteomic studies of the Arabidopsis TRAPP complexes reveal conserved organization and a novel plant-specific component with a role in plant development
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拟南芥 TRAPP 复合物的蛋白质组学研究揭示了保守的组织和在植物发育中发挥作用的新型植物特异性成分
DOI：
10.1101/684258
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Garcia V
中科院分区：
文献类型：
--
作者：
Garcia V
How the membrane trafficking system spatially organizes intracellular activities and intercellular signaling networks is not well understood in plants. The Transport Protein Particle (TRAPP) complexes are known to play key roles in selective delivery of membrane vesicles to various subcellular compartments in yeast and animals, but remain to be fully characterized in plants. Here we interrogate the TRAPP complexes in Arabidopsis using quantitative proteomic approaches. TRS33 is a component shared by all TRAPP complexes in yeast and animals, and the Arabidopsis AtTRS33 is essential for the subcellular dynamics of other TRAPP components. Affinity purification of AtTRS33 followed by quantitative mass spectrometry identified fourteen interacting proteins; these include not only thirteen homologs of all known TRAPP components in yeast and mammals but also a novel protein we named TRAPP-interacting plant protein (TRIPP), which is conserved in multi-cellular photosynthetic organisms. Proteomic and molecular analyses showed that TRIPP specifically associates with the TRAPPII complexin vivoand directly interacts with the TRAPPII-specific subunits but not the subunits shared with TRAPPIII. TRIPP co-localizes with a subset of TRS33 compartments, and its localization is disrupted in thetrs33mutant. Loss-of-functiontrippmutation caused growth and reproductive development defects, including partial photomorphogenesis in the dark. Our study demonstrates that plants possess at least two distinct TRAPP complexes similar to metazoan, and identifies TRIPP as a novel plant-specific component of the TRAPPII complex with important functions in plant growth and development.
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影响因子：
3.3
作者：
A. Yamasaki;Shekar Menon;Sidney Yu;Jemima Barrowman;T. Meerloo;V. Oorschot;J. Klumperman;Ayano Satoh;S. Ferro-Novick
通讯作者：
A. Yamasaki;Shekar Menon;Sidney Yu;Jemima Barrowman;T. Meerloo;V. Oorschot;J. Klumperman;Ayano Satoh;S. Ferro-Novick
影响因子：
3.3
作者：
P. James;J. Halladay;E. Craig
通讯作者：
P. James;J. Halladay;E. Craig
DOI：
10.15252/embj.201592695
发表时间：
2016-02-01
期刊：
The EMBO journal
影响因子：
--
作者：
Lamb CA;Nühlen S;Judith D;Frith D;Snijders AP;Behrends C;Tooze SA
通讯作者：
Tooze SA
影响因子：
4.6
作者：
Zhao S;Li CM;Luo XM;Siu GK;Gan WJ;Zhang L;Wu WK;Chan HC;Yu S
通讯作者：
Yu S
影响因子：
11.8
作者：
Rybak, Katarzyna;Steiner, Alexander;Assaad, Farhah F.
通讯作者：
Assaad, Farhah F.