Time-Dependent Impact of Irreversible Electroporation on Pancreas, Liver, Blood Vessels and Nerves: A Systematic Review of Experimental Studies.
Time-Dependent Impact of Irreversible Electroporation on Pancreas, Liver, Blood Vessels and Nerves: A Systematic Review of Experimental Studies.
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DOI：
10.1371/journal.pone.0166987
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Besselink MG
中科院分区：
文献类型：
--
作者：
Vogel JA;van Veldhuisen E;Agnass P;Crezee J;Dijk F;Verheij J;van Gulik TM;Meijerink MR;Vroomen LG;van Lienden KP;Besselink MG
不可逆电穿孔（IRE）是一种治疗不可切除癌症的新型消融技术。与热技术中的坏死相比，非热机制被认为主要引起细胞凋亡。在实验和临床研究中，经常报告消融和组织或成像分析之间的等待时间，以允许通过细胞凋亡进行细胞死亡。然而，随着时间的推移，IRE效应的动态仍然未知。因此，本研究旨在总结这些影响与治疗和评估之间的时间。在Pubmed、Embase和科克伦图书馆中进行了系统检索，以查找在动物或人体研究中使用IRE对胰腺、肝脏或周围结构进行研究的原始文献。提取病理学数据和IRE与评价之间的时间。在2602项筛选的研究中，36项可纳入，涉及超过440只动物（猪、大鼠、山羊和兔）的肝脏（n = 24）、胰腺（n = 4）、血管（n = 4）和神经（n = 4）中的IRE。在肝脏和胰腺中，在治疗后1-2小时观察到细胞凋亡和出血的最初迹象，并且在肝脏中保持可见直到24小时，在胰腺中保持可见直到7天，之后受损组织被纤维化取代。在孤立血管中，图尼卡中膜、内膜和管腔在24小时内保持不变。7天后，表现出炎症、纤维化和平滑肌细胞损失，其持续至35天。在神经中，直到明显的组织学变化的中位时间为7天。IRE后的组织损伤是一个动态过程，组织间存在明显的时间差。胰腺和肝脏在1-2小时后出现第一次损伤，而血管需要24小时，神经需要7天。
Irreversible electroporation (IRE) is a novel ablation technique in the treatment of unresectable cancer. The non-thermal mechanism is thought to cause mostly apoptosis compared to necrosis in thermal techniques. Both in experimental and clinical studies, a waiting time between ablation and tissue or imaging analysis to allow for cell death through apoptosis, is often reported. However, the dynamics of the IRE effect over time remain unknown. Therefore, this study aims to summarize these effects in relation to the time between treatment and evaluation. A systematic search was performed in Pubmed, Embase and the Cochrane Library for original articles using IRE on pancreas, liver or surrounding structures in animal or human studies. Data on pathology and time between IRE and evaluation were extracted. Of 2602 screened studies, 36 could be included, regarding IRE in liver (n = 24), pancreas (n = 4), blood vessels (n = 4) and nerves (n = 4) in over 440 animals (pig, rat, goat and rabbit). No eligible human studies were found. In liver and pancreas, the first signs of apoptosis and haemorrhage were observed 1–2 hours after treatment, and remained visible until 24 hours in liver and 7 days in pancreas after which the damaged tissue was replaced by fibrosis. In solitary blood vessels, the tunica media, intima and lumen remained unchanged for 24 hours. After 7 days, inflammation, fibrosis and loss of smooth muscle cells were demonstrated, which persisted until 35 days. In nerves, the median time until demonstrable histological changes was 7 days. Tissue damage after IRE is a dynamic process with remarkable time differences between tissues in animals. Whereas pancreas and liver showed the first damages after 1–2 hours, this took 24 hours in blood vessels and 7 days in nerves.
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影响因子：
11.2
作者：
Guo Y;Zhang Y;Klein R;Nijm GM;Sahakian AV;Omary RA;Yang GY;Larson AC
通讯作者：
Larson AC
影响因子：
3.7
作者：
Li W;Zhu S;Li J;Huang Y;Zhou R;Fan X;Yang H;Gong X;Eissa NT;Jahnen-Dechent W;Wang P;Tracey KJ;Sama AE;Wang H
通讯作者：
Wang H
影响因子：
39.2
作者：
Hutton, Brian;Salanti, Georgia;Moher, David
通讯作者：
Moher, David
影响因子：
4.6
作者：
Edd, Jon F.;Horowitz, Liana;Rubinsky, Boris
通讯作者：
Rubinsky, Boris
影响因子：
4.6
作者：
Golberg, Alexander;Yarmush, Martin L.
通讯作者：
Yarmush, Martin L.