Delayed clinical and ex vivo response to mammalian meat in patients with IgE to galactose-alpha-1,3-galactose.
Delayed clinical and ex vivo response to mammalian meat in patients with IgE to galactose-alpha-1,3-galactose.
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DOI：
10.1016/j.jaci.2014.01.024
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发表时间：
2014-07
影响因子：
14.2
通讯作者：
Platts-Mills, Thomas A. E.
中科院分区：
文献类型：
--
作者：
Commins, Scott P.;James, Hayley R.;Stevens, Whitney;Pochan, Shawna L.;Land, Michael H.;King, Carol;Mozzicato, Susan;Platts-Mills, Thomas A. E.
在2009年，我们报告了一种新的形式的迟发性过敏反应，红肉相关的血清IgE抗体的寡糖半乳糖-α-1，3-半乳糖（α-半乳糖）。虽然患者在他们的描述中非常一致，即在食用哺乳动物肉类和出现症状之间有3至6小时的延迟，但这种延迟尚未在观察研究中得到证实。我们试图正式记录摄入哺乳动物肉类后，IgE α-半乳糖的受试者的临床症状的时间过程，并监测体外过敏反应的标志物的外观。在12名有严重荨麻疹反应史的受试者中，在食用牛肉、猪肉或羊肉后3至6小时，以及13名对照受试者中，用哺乳动物肉进行开放式食物挑战。每次攻毒期间每小时采集一次血样。12名对α-gal有IgE的受试者中有10名在食物激发期间有反应的临床证据（与对照受试者相比，没有，P <0.001）。这些反应发生在最初摄入哺乳动物肉后3至7小时，范围从荨麻疹到过敏反应。在3次攻毒中类胰蛋白酶水平呈阳性。嗜碱性粒细胞活化，如通过CD63表达增加所测量的，与临床症状的出现相关。结果提供了明确的证据，IgE介导的食物过敏发生后几个小时摄入的煽动过敏原。此外，在这里，我们报告说，在体内嗜碱性粒细胞激活过程中的食物挑战发生在相同的时间框架的临床症状，并可能反映了抗原在血流中的外观。（J Allergy Clin Immunol 2014;）
In 2009, we reported a novel form of delayed anaphylaxis to red meat related to serum IgE antibodies to the oligosaccharide galactose-alpha-1,3-galactose (alpha-gal). Although patients were remarkably consistent in their description of a 3- to 6-hour delay between eating mammalian meat and the appearance of symptoms, this delay has not been demonstrated under observed studies. We sought to formally document the time course of clinical symptoms after the ingestion of mammalian meat in subjects with IgE to alpha-gal and to monitor ex vivo for the appearance of markers of an allergic reaction. Open food challenges were performed with mammalian meat in 12 subjects with a history of severe urticarial reactions 3 to 6 hours after eating beef, pork, or lamb, as well as in 13 control subjects. Blood samples were taken hourly during each challenge. Ten of 12 subjects with IgE to alpha-gal had clinical evidence of a reaction during the food challenge (vs none of the control subjects, P < .001). The reactions occurred 3 to 7 hours after the initial ingestion of mammalian meat and ranged from urticaria to anaphylaxis. Tryptase levels were positive in 3 challenges. Basophil activation, as measured by increased expression of CD63, correlated with the appearance of clinical symptoms. The results presented provide clear evidence of an IgE-mediated food allergy that occurs several hours after ingestion of the inciting allergen. Moreover, here we report that in vivo basophil activation during a food challenge occurs in the same time frame as clinical symptoms and likely reflects the appearance of the antigen in the bloodstream. (J Allergy Clin Immunol 2014;)
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影响因子：
14.2
作者：
Erwin, EA;Custis, NJ;Platts-Mills, TAE
通讯作者：
Platts-Mills, TAE
DOI：
10.1016/0005-2760(87)90121-4
发表时间：
1987-02-23
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
GONEN, B;ODONNELL, P;SCHULMAN, ES
通讯作者：
SCHULMAN, ES
影响因子：
2.8
作者：
Platz, IJ;Binder, M;Bühring, HJ
通讯作者：
Bühring, HJ
DOI：
10.1073/pnas.0610012104
发表时间：
2007-01-09
影响因子：
11.1
作者：
Koike, Chihiro;Uddin, Monica;Goodman, Morris
通讯作者：
Goodman, Morris
DOI：
10.1016/j.jaci.2011.02.019
发表时间：
2011-05
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Commins SP;James HR;Kelly LA;Pochan SL;Workman LJ;Perzanowski MS;Kocan KM;Fahy JV;Nganga LW;Ronmark E;Cooper PJ;Platts-Mills TA
通讯作者：
Platts-Mills TA