Survival of Vibrio cholerae in nutrient-poor environments is associated with a novel "persister" phenotype.
Survival of Vibrio cholerae in nutrient-poor environments is associated with a novel "persister" phenotype.
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DOI：
10.1371/journal.pone.0045187
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Ali A
中科院分区：
文献类型：
--
作者：
Jubair M;Morris JG Jr;Ali A
为了应对抗生素和/或环境压力，一些细菌物种转变为“持久”表型。虽然致病性霍乱弧菌，负责霍乱，可以发现在营养不良的水生环境中流行的地区，可培养的细胞坚持在这些环境水库的基本机制（S）是未知的。在这里，我们报道了将霍乱弧菌引入营养贫乏的过滤灭菌湖水（FSLW）微观世界中促进了向我们定义为可培养>700天的“持留”表型（PP）的转变。将霍乱弧菌的PP从原始微观世界直接转移到新鲜制备的FSLW导致在原始微观世界中观察到的相同的持久性模式。持续700多天的细胞扫描电子显微镜显示细胞形态非常小，具有与从细胞的各个方面发出的鞭毛相关的高度聚集。当转移到营养丰富的L-肉汤中时，霍乱弧菌PP细胞恢复为典型的霍乱弧菌形态。在24小时、180天和700天时从微宇宙获得的无细胞上清液均显示CAI-1信号传导分子增加>2倍，这与群体感应活性一致，如已经针对铜绿假单胞菌持留细胞所描述的。几丁质和磷酸盐促进细胞生长。我们的数据表明，营养压力可以选择一个V. cholecysterpersister表型在环境水库，这些菌株，然后播种随后的霍乱疫情响应几丁质和磷酸盐的可用性。
In response to antibiotic and/or environmental stress, some species of bacteria shift to a “persister” phenotype. Although toxigenic Vibrio cholerae, responsible for the disease cholera, can be found in nutrient-poor aquatic environments in endemic areas, the underlying mechanism(s) by which culturable cells persist in these environmental reservoirs is largely unknown. Here we report that introduction of V. cholerae into a nutrient-poor filter sterilized lake water (FSLW) microcosm promoted a shift to what we have defined as a “persister” phenotype (PP) which was culturable for >700 days. Direct transfer of PP of V. cholerae from original microcosms to freshly prepared FSLW resulted in the same pattern of persistence seen in the original microcosms. Scanning electron microscopy of cells persisting for over 700 days demonstrated cell morphologies that were very small in size, with a high degree of aggregation associated with flagella emanating from all aspects of the cell. V. cholerae PP cells reverted to a typical V. cholerae morphology when transferred to nutrient-rich L- broth. Cell-free supernatants obtained from microcosms at 24 hours, 180 days, and 700 days all showed >2-fold increase in CAI-1 signaling molecules, consistent with quorum sensing activity, as has been described for Pseudomonas aeruginosa persister cells. Chitin and phosphate promoted cell growth. Our data suggest that nutrient stress can select a V. cholerae persister phenotype in environmental reservoirs, with these strains then seeding subsequent cholera epidemics in response to chitin and phosphate availability.
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