The elucidation of stress memory inheritance in Brassica rapa plants.
The elucidation of stress memory inheritance in Brassica rapa plants.
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DOI：
10.3389/fpls.2015.00005
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发表时间：
2015
影响因子：
5.6
通讯作者：
Kovalchuk I
中科院分区：
文献类型：
--
作者：
Bilichak A;Ilnytskyy Y;Wóycicki R;Kepeshchuk N;Fogen D;Kovalchuk I
植物能够在整个个体发生过程中保持对压力暴露的记忆，并将其忠实地传播到下一代。最近的证据支持这种现象的表观遗传本质。小RNA (smRNA) 是重要的表观遗传因素之一，因为它们既可以影响其生成位置的基因表达，又可以维持非细胞自主基因调控。在这里，我们尝试利用测序技术破译smRNA对白菜植物热休克诱导的跨代遗传的贡献。为此，我们从受胁迫植物及其未经处理的后代的体细胞和生殖组织中生成了转录组和小 RNAome (smRNAome) 的全面概况。我们已经证明，在未直接暴露于应激的组织（即胚乳和花粉）中检测到转录组和 smRNAome 谱的最高组织特异性改变。重要的是，我们发现，胁迫植物的后代在 smRNAome 水平上表现出最高的波动，但在转录组水平上则不然。此外，我们还发现植物中存在热诱导和跨代传递的 tRNA 衍生小 RNA 片段。最后，我们认为 miR168 和 braAGO1 参与植物胁迫诱导的跨代遗传。
Plants are able to maintain the memory of stress exposure throughout their ontogenesis and faithfully propagate it into the next generation. Recent evidence argues for the epigenetic nature of this phenomenon. Small RNAs (smRNAs) are one of the vital epigenetic factors because they can both affect gene expression at the place of their generation and maintain non-cell-autonomous gene regulation. Here, we have made an attempt to decipher the contribution of smRNAs to the heat-shock-induced transgenerational inheritance in Brassica rapa plants using sequencing technology. To do this, we have generated comprehensive profiles of a transcriptome and a small RNAome (smRNAome) from somatic and reproductive tissues of stressed plants and their untreated progeny. We have demonstrated that the highest tissue-specific alterations in the transcriptome and smRNAome profile are detected in tissues that were not directly exposed to stress, namely, in the endosperm and pollen. Importantly, we have revealed that the progeny of stressed plants exhibit the highest fluctuations at the smRNAome level but not at the transcriptome level. Additionally, we have uncovered the existence of heat-inducible and transgenerationally transmitted tRNA-derived small RNA fragments in plants. Finally, we suggest that miR168 and braAGO1 are involved in the stress-induced transgenerational inheritance in plants.
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影响因子：
3.7
作者：
Boyko A;Blevins T;Yao Y;Golubov A;Bilichak A;Ilnytskyy Y;Hollunder J;Meins F Jr;Kovalchuk I
通讯作者：
Kovalchuk I
影响因子：
14.9
作者：
Dai X;Zhao PX
通讯作者：
Zhao PX
影响因子：
3.7
作者：
Fahlgren N;Howell MD;Kasschau KD;Chapman EJ;Sullivan CM;Cumbie JS;Givan SA;Law TF;Grant SR;Dangl JL;Carrington JC
通讯作者：
Carrington JC
影响因子：
64.5
作者：
Calarco JP;Borges F;Donoghue MT;Van Ex F;Jullien PE;Lopes T;Gardner R;Berger F;Feijó JA;Becker JD;Martienssen RA
通讯作者：
Martienssen RA
影响因子：
6.9
作者：
DAUGHERTY, CJ;MUSGRAVE, ME
通讯作者：
MUSGRAVE, ME