Concentrated collagen-chondroitin sulfate scaffolds for tissue engineering applications.
Concentrated collagen-chondroitin sulfate scaffolds for tissue engineering applications.
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DOI：
10.1002/jbm.a.32774
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发表时间：
2010-09-15
影响因子：
4.9
通讯作者：
Baskaran, Harihara
中科院分区：
文献类型：
--
作者：
Liang, Wan-Hsiang;Kienitz, Brian L.;Penick, Kitsie J.;Welter, Jean F.;Zawodzinski, Thomas A.;Baskaran, Harihara
关键词：
胶原蛋白-硫酸软骨素生物材料支架已用于许多正在开发或临床的组织工程产品。在本文中，我们描述了一种基于离心的新方法，用于获得高度浓缩且多孔的胶原蛋白支架。研究了浓缩支架的吸水率、硫酸软骨素保留率、形态、机械性能和组织工程潜力。我们的结果表明，新方法可以使支架含有的胶原蛋白是传统非浓缩支架的四倍。此外，浓缩支架中的水吸收显着更高，而硫酸软骨素保留在浓缩支架中不受影响。浓缩支架中的平均孔径值小于未浓缩支架中的平均孔径值，并且前者的孔壁由连续的胶原片组成。在浓缩支架中，以压缩和拉伸弹性模量衡量的机械性能提高了 30 倍。此外，我们对人类间充质干细胞和包皮角质形成细胞的组织培养结果表明，新支架可用于软骨和皮肤组织工程应用。
Collagen-chondroitin sulfate biomaterial scaffolds have been used in a number of tissue engineered products under development or in the clinics. In this paper, we describe a new approach based on centrifugation for obtaining highly concentrated yet porous collagen scaffolds. Water uptake, chondroitin sulfate retention, morphology, mechanical properties and tissue engineering potential of the concentrated scaffolds were investigated. Our results show that the new approach can lead to scaffolds containing four times as much collagen as that in conventional unconcentrated scaffolds. Further, water uptake in the concentrated scaffolds was significantly greater while chondroitin sulfate retention in the concentrated scaffolds was unaffected. The value of mean pore diameter in the concentrated scaffolds was smaller than that in the unconcentrated scaffolds and the walls of the pores in the former comprised of a continuous sheet of collagen. The mechanical properties measured as moduli of elasticity in compression and tension were improved by as much as 30 times in the concentrated scaffolds. In addition, our tissue culture results with human mesenchymal stem cells and foreskin keratinocytes show that the new scaffolds can be used for cartilage and skin tissue engineering applications.
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DOI：
10.1515/bmte.2002.47.s1a.485
发表时间：
2002-01-01
期刊：
Biomedizinische Technik. Biomedical engineering
影响因子：
--
作者：
Kuberka, M;Heschel, I;Rau, G
通讯作者：
Rau, G
影响因子：
2.8
作者：
Buijtenhuijs, P;Buttafoco, L;Vermes, I
通讯作者：
Vermes, I
影响因子：
9.7
作者：
Harley, Brendan A.;Leung, Janet H.;Gibson, Lorna J.
通讯作者：
Gibson, Lorna J.
影响因子：
3.2
作者：
Eaglstein, WH;Falanga, V
通讯作者：
Falanga, V
影响因子：
4.9
作者：
Orban, JM;Wilson, LB;Vorp, DA
通讯作者：
Vorp, DA