Improvement of Astaxanthin Production in Aurantiochytrium limacinum by Overexpression of the Beta-Carotene Hydroxylase Gene
Improvement of Astaxanthin Production in Aurantiochytrium limacinum by Overexpression of the Beta-Carotene Hydroxylase Gene
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通过过度表达 β-胡萝卜素羟化酶基因提高 Aurantiochytrium limacinum 中虾青素的产量
DOI：
10.1007/s12010-022-04172-4
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发表时间：
2022
影响因子：
3
通讯作者：
Sawayama Shigeki
中科院分区：
文献类型：
--
作者：
Yoshimi Toru;Hashimoto Sakiko;Kubo Yuki;Takeuchi Masato;Morimoto Daichi;Nakagawa Satoshi;Sawayama Shigeki
桔黄壶菌是一种异养真核微生物，能够积累大量的商业产品，如虾青素和二十二碳六烯酸。由于其生长速度快且提取方法相对简单，A.limacinumis被认为是一种很有前途的虾青素资源，可以取代传统的微藻生产。然而，对A. limacinum中虾青素的生物合成过程，特别是β-胡萝卜素羟化酶（CrtZ）和酮醇酶催化的过程，仍不完全清楚。在这项研究中，我们过表达acrtZ候选基因，以增加虾青素的生产和扩大我们的理解从β-胡萝卜素到虾青素的转换。与野生型菌株相比，培养5天的所得菌株AlcrtZ#10显示每培养物（2.8倍）和每细胞（4.5倍）的虾青素产量显著增加。引人注目的是，较长的光照时间增加了野生型菌株中的虾青素产量，并降低了β-胡萝卜素含量，这表明光照时间对A. limacinum的虾青素生产很重要。此外，在几种预测的中间体中，通过酮醇酶活性由β-胡萝卜素产生的坎他黄素在A1crtZ#10中增强。虽然还需要进一步的研究，但这一结果表明，虾青素的主要途径是通过角黄素。因此，我们的研究结果将是有价值的，不仅为它的应用，但也了解虾青素的生物合成过程中A. limacinum。
Aurantiochytrium limacinumis a heterotrophic eukaryotic microorganism that can accumulate high levels of commercial products such as astaxanthin and docosahexaenoic acid. Due to its rapid growth and relatively simple extraction method,A.limacinumis considered a promising astaxanthin resource to replace the conventional microalgal production. However, the astaxanthin biosynthetic process inA.limacinumremains incompletely understood, especially in those catalysed by β-carotene hydroxylase (CrtZ) and ketolase. In this study, we overexpressed acrtZcandidate gene to increase astaxanthin production and expand our understanding of the conversion from beta-carotene to astaxanthin. The resultant transformant AlcrtZ#10 cultivated for 5 days showed a significant increase in astaxanthin production per culture (2.8-fold) and per cell (4.5-fold) compared with that of the wild-type strain. Strikingly, longer light exposure increased astaxanthin production and decreased the beta-carotene content in the wild-type strain, suggesting that light exposure duration is important for astaxanthin production inA.limacinum. Among several predicted intermediates, furthermore, the cantaxanthin produced from β-carotene by ketolase activity were enhanced in the transformant AlcrtZ#10. Although the further investigation is needed, this result suggested that the main route of astaxanthin was via cantaxanthin. Thus, our findings will be valuable not only for its application, but also for understanding the astaxanthin biosynthetic process inA.limacinum.
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影响因子：
4
作者：
Yuki Kubo;D. Morimoto;M. Shiroi;Toru Yoshimi;Kanta Ohara;Tokuhiro Higashine;Yuki Mori;Masato Takeuchi;S. Sawayama
通讯作者：
Yuki Kubo;D. Morimoto;M. Shiroi;Toru Yoshimi;Kanta Ohara;Tokuhiro Higashine;Yuki Mori;Masato Takeuchi;S. Sawayama
使用化学发光检测系统测定常见亲水性和亲脂性抗氧化剂对单线态氧的猝灭活性
DOI：
--
发表时间：
2007
期刊：
--
影响因子：
--
作者：
Y. Nishida;E. Yamashita
通讯作者：
E. Yamashita
破囊壶菌生产类胡萝卜素的条件。
影响因子：
1.6
作者：
V. J. Furlan;I. Batista;N. Bandarra;R. Mendes;C. Cardoso
通讯作者：
C. Cardoso
影响因子：
2.7
作者：
Moran, C. A.;Morlacchini, M.;Fusconi, G.
通讯作者：
Fusconi, G.
影响因子：
4.5
作者：
Nobrega, Renata Oselame;Batista, Rosana Oliveira;Fracalossi, Debora Machado
通讯作者：
Fracalossi, Debora Machado