Tumor-induced lymphangiogenesis in cervical lymph nodes in oral melanoma-bearing mice.
Tumor-induced lymphangiogenesis in cervical lymph nodes in oral melanoma-bearing mice.
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DOI：
10.1186/1756-9966-31-83
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发表时间：
2012-10-02
期刊：
影响因子：
--
通讯作者：
Taniguchi K
中科院分区：
文献类型：
--
作者：
Ozasa R;Ohno J;Iwahashi T;Taniguchi K
通过淋巴系统的转移由淋巴管生成促进。在转移到区域淋巴结（LN）的进展过程中，淋巴管的变化仍然没有探索。为了研究肿瘤诱导的淋巴结淋巴管生成是否控制局部淋巴结转移，我们研究了小鼠口腔黑色素瘤模型的颈部淋巴结转移。将B16 F10黑色素瘤细胞注射到小鼠舌中复制自发性颈淋巴结转移。我们对肿瘤相关淋巴结中的肿瘤反应性淋巴结病和淋巴管生成进行了组织学、免疫荧光和组织形态学分析。我们研究了血管内皮生长因子（VEGF）-C和它的受体，VEGF受体-3（VEGFR-3），在肿瘤细胞和组织中的表达，并通过逆转录聚合酶链反应和免疫荧光。肿瘤相关淋巴结包括肿瘤细胞浸润前后的前哨淋巴结（SLN）（带瘤SLN）以及与带瘤SLN相邻或对侧的前哨淋巴结。SLN在转移前出现广泛的淋巴管生成。在SLN中建立转移后，邻近的和对侧的SLN均显示淋巴管生成。VEGF-C阳性黑色素瘤细胞和VEGFR-3阳性淋巴管之间的相互作用在肿瘤相关淋巴结中是明显的。LN淋巴管生成有助于肿瘤从SLN向其他区域LN转移的进展。
Metastasis via the lymphatic system is promoted by lymphangiogenesis. Alterations of the lymphatic channels during the progression of metastasis to regional lymph nodes (LNs) remain unexplored. To examine whether tumor-induced LN lymphangiogenesis controls metastasis to regional LNs, we investigated cervical LN metastasis in a mouse model of oral melanoma. Injection of B16F10 melanoma cells into mouse tongues replicated spontaneous cervical LN metastasis. We performed histological, immunofluorescent, and histomorphometric analyses of tumor-reactive lymphadenopathy and lymphangiogenesis in tumor-associated LNs. We investigated the expression of vascular endothelial growth factor (VEGF)-C and its receptor, VEGF receptor-3 (VEGFR-3), in tumor cells and tissues, and LNs by reverse transcription polymerase chain reaction and immunofluorescence. Tumor-associated LNs comprised sentinel LNs (SLNs) before and after tumor cell invasion (tumor-bearing SLNs), and LNs adjacent or contralateral to tumor-bearing SLNs. Extensive lymphangiogenesis appeared in SLNs before evidence of metastasis. After metastasis was established in SLNs, both LNs adjacent and contralateral to tumor-bearing SLNs demonstrated lymphangiogenesis. Interaction between VEGF-C-positive melanoma cells and VEGFR-3-positive lymphatic vessels was evident in tumor-associated LNs. LN lymphangiogenesis contributes a progression of tumor metastasis from SLNs to other regional LNs.
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影响因子：
82.9
作者：
Skobe, M;Hawighorst, T;Detmar, M
通讯作者：
Detmar, M
DOI：
10.1093/jnci/94.11.819
发表时间：
2002-06-05
期刊：
JOURNAL OF THE NATIONAL CANCER INSTITUTE
影响因子：
--
作者：
He, YL;Kozaki, KI;Alitalo, K
通讯作者：
Alitalo, K
影响因子：
20.3
作者：
Hirakawa, Satoshi;Brown, Lawrence F.;Detmar, Michael
通讯作者：
Detmar, Michael
影响因子：
16.8
作者：
Jackson, DG;Prevo, R;Banerji, S
通讯作者：
Banerji, S
影响因子：
7.8
作者：
Banerji, S;Ni, J;Wang, S X;Clasper, S;Su, J;Tammi, R;Jones, M;Jackson, D G
通讯作者：
Jackson, D G