Extracorporeal shock wave therapy inhibits osteoclast differentiation by targeting NF-κB signaling pathway.
Extracorporeal shock wave therapy inhibits osteoclast differentiation by targeting NF-κB signaling pathway.
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DOI：
10.1186/s13018-023-04166-w
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发表时间：
2023-10-27
影响因子：
2.6
通讯作者：
Li, Baofeng
中科院分区：
文献类型：
--
作者：
Chen, Bei;Luo, Yeqiang;Zhang, Zhongxiu;Lin, Shanghui;Wang, Renkai;Li, Baofeng
体外冲击波治疗（ESWT）已被报道促进成骨细胞分化。然而，体外冲击波治疗对破骨细胞分化的作用仍然是难以捉摸的。本研究通过TRAP染色、RT-PCR、WB法、MTT法等方法对冲击波组和对照组体外培养的破骨细胞的分化情况进行分析。此外，我们分析了这两组在体内的骨形成，并评估了两组之间的micro-CT和trap染色。我们发现体外ESWT抑制破骨细胞的成熟，体内ESW处理股骨促进骨形成。机械地，破骨细胞的分化受到抑制的冲动的数量增加和ESWT降低NTAFc 1和P65蛋白的内源性水平。体外冲击波治疗可能通过NF-κB信号通路治疗骨质疏松症。
Extracorporeal shock wave therapy (ESWT) has been reported to promote osteoblast differentiation. However, the role of ESWT on osteoclast differentiation is still elusive. This study analyzed the differentiation of osteoclasts in the shock wave group and the control group in vitro, and TRAP staining, RT-PCR, WB assays, and MTT assays were assessed between the two groups. Furthermore, we analyzed the bone formation in these two groups in vivo and micro-CT and trap staining were assessed between the two groups. We found that ESWT inhibited osteoclast maturation in vitro and ESW treatment of femur promoted bone formation in vivo. Mechanically, osteoclast differentiation was inhibited as the number of impulses increased and ESWT decreased endogenous levels of NTAFc1 and P65 protein. ESWT may be a potential therapy of osteoporosis through NF-κB signaling pathway.
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影响因子：
6.7
作者：
Schmitz C;Császár NB;Milz S;Schieker M;Maffulli N;Rompe JD;Furia JP
通讯作者：
Furia JP
DOI：
10.1097/00003086-200106000-00007
发表时间：
2001-06-01
影响因子：
4.2
作者：
Ogden, JA;Alvarez, R;Marlow, M
通讯作者：
Marlow, M
影响因子：
2.6
作者：
Wu W;Maffulli N;Furia JP;Meindlhumer L;Sternecker K;Milz S;Schmitz C
通讯作者：
Schmitz C
影响因子：
5.2
作者：
Zhang H;Wang R;Wang G;Zhang B;Wang C;Li D;Ding C;Wei Q;Fan Z;Tang H;Ji F
通讯作者：
Ji F
影响因子：
4.2
作者：
Schleusser, Sophie;Song, Jungin;Kisch, Tobias
通讯作者：
Kisch, Tobias