Exposure of zebra mussels to radial extracorporeal shock waves: implications for treatment of fracture nonunions.
Exposure of zebra mussels to radial extracorporeal shock waves: implications for treatment of fracture nonunions.
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斑马贻贝暴露于放射状体外冲击波：骨折不愈合治疗的意义。
DOI：
10.1186/s13018-021-02852-1
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发表时间：
2021-12-04
影响因子：
2.6
通讯作者：
Schmitz C
中科院分区：
文献类型：
--
作者：
Wu W;Maffulli N;Furia JP;Meindlhumer L;Sternecker K;Milz S;Schmitz C
径向体外冲击波治疗（rESWT）是一种有吸引力的非侵入性治疗选择，用于治疗浅表骨骨折不愈合，据报道成功率约为75%。使用斑马贻贝（Dreissena polymorpha），我们最近证明，生物矿化的诱导后，暴露于集中体外冲击波（fESW）不限于该地区的直接能量转移到钙化组织。本研究验证了这样一个假设，即径向体外冲击波（rESW）也会在斑马贻贝中不直接暴露于冲击波能量的区域诱导生物矿化。将斑马贻贝的左瓣暴露于不同气压（0至4巴）下的1000个rESW，然后在钙黄绿素溶液中孵育24小时。通过研究在暴露后两周制备的左右瓣膜切片上发现的荧光信号强度来评价生物矿化。一般线性模型分析显示有统计学意义（p <0.05）施加的冲击波能量以及侧面的影响（左侧/暴露与右侧/未暴露）和瓣膜的研究区域（在曝光位置相对于离曝光一定距离的位置）的平均荧光信号强度值，以及统计学上显著的能量×区域和能量×侧×区域组合效应。最高的平均荧光信号强度值发现在脐附近，即，不在直接暴露于rESW的位置。与fESW的应用一样，通过暴露于rESW诱导生物矿化可能不限于直接能量转移到钙化组织中的区域。此外，本研究的结果可能有助于更好地理解为什么在使用体外冲击波治疗骨折不愈合时，应用超过一定阈值的更高能量通量密度不一定会导致更高的成功率。
Radial extracorporeal shock wave therapy (rESWT) is an attractive, non-invasive therapy option to manage fracture nonunions of superficial bones, with a reported success rate of approximately 75%. Using zebra mussels (Dreissena polymorpha), we recently demonstrated that induction of biomineralization after exposure to focused extracorporeal shock waves (fESWs) is not restricted to the region of direct energy transfer into calcified tissue. This study tested the hypothesis that radial extracorporeal shock waves (rESWs) also induce biomineralization in regions not directly exposed to the shock wave energy in zebra mussels. Zebra mussels were exposed on the left valve to 1000 rESWs at different air pressure (between 0 and 4 bar), followed by incubation in calcein solution for 24 h. Biomineralization was evaluated by investigating the fluorescence signal intensity found on sections of the left and right valves prepared two weeks after exposure. General linear model analysis demonstrated statistically significant (p < 0.05) effects of the applied shock wave energy as well as of the side (left/exposed vs. right/unexposed) and the investigated region of the valve (at the position of exposure vs. positions at a distance to the exposure) on the mean fluorescence signal intensity values, as well as statistically significant combined energy × region and energy × side × region effects. The highest mean fluorescence signal intensity value was found next to the umbo, i.e., not at the position of direct exposure to rESWs. As in the application of fESWs, induction of biomineralization by exposure to rESWs may not be restricted to the region of direct energy transfer into calcified tissue. Furthermore, the results of this study may contribute to better understand why the application of higher energy flux densities beyond a certain threshold does not necessarily lead to higher success rates when treating fracture nonunions with extracorporeal shock wave therapy.
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影响因子：
3.7
作者：
Császár NB;Angstman NB;Milz S;Sprecher CM;Kobel P;Farhat M;Furia JP;Schmitz C
通讯作者：
Schmitz C
影响因子：
4.6
作者：
Hochstrasser T;Frank HG;Schmitz C
通讯作者：
Schmitz C
影响因子：
5.3
作者：
Cacchio, Angelo;Giordano, Lucio;Santilli, Valter
通讯作者：
Santilli, Valter
影响因子：
6.7
作者：
Schmitz C;Császár NB;Milz S;Schieker M;Maffulli N;Rompe JD;Furia JP
通讯作者：
Furia JP
影响因子：
2.9
作者：
Gollwitzer, Hans;Gloeck, Timo;Diehl, Peter
通讯作者：
Diehl, Peter