Members of the barley NAC transcription factor gene family show differential co-regulation with senescence-associated genes during senescence of flag leaves.
Members of the barley NAC transcription factor gene family show differential co-regulation with senescence-associated genes during senescence of flag leaves.
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DOI：
10.1093/jxb/eru046
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发表时间：
2014-07
影响因子：
6.9
通讯作者：
Gregersen PL
中科院分区：
文献类型：
--
作者：
Christiansen MW;Gregersen PL
大麦NAC转录因子家族成员通过调控启动子中含有回文NAC结合序列的靶基因，高度参与叶片衰老的发生和发展。植物的衰老过程对其生命周期的完成至关重要，特别是对农作物来说，它是种子灌浆过程中有效的营养物质再动员的关键。这是一个高度调控的过程，为了解决调控方面的问题，我们研究了NAC转录因子家族中的基因在大麦旗叶衰老过程中的作用。在基因芯片实验中，NAC转录因子基因家族的几个成员在衰老过程中上调，以及一系列与衰老相关的基因，反映了衰老大麦叶片组织中降解过程的协调激活。这一图景在详细的定量逆转录-聚合酶链式反应(qRT-PCR)实验中得到了证实，该实验还显示了NAC基因家族不同成员的不同基因表达模式，表明在47个已研究的NAC基因中，有15个基因在大麦叶片衰老过程中对信号传递过程和降解过程的执行具有重要作用。在已发表的NAC转录因子DNA结合基序的基础上，设计了7个NAC转录因子DNA结合基序模型，并对大麦基因启动子序列进行了筛选。在衰老过程中上调的基因显示出显著的过度表达，这表明NAC转录因子的调控。此外，共调控研究表明，具有启动子基序的基因通常与NAC基因家族成员高度共表达。综上所述，来自大麦的多达15个NAC基因是影响谷类作物产量的衰老和生物胁迫相关性状的重要调控因子的有力候选者。此外，还列出了71个与衰老相关的基因，它们是这些NAC转录因子的潜在靶基因。
Members of the NAC transcription factor family in barley appear to be highly involved in initiation and progression of leaf senescence via regulation of target genes having palindromic NAC-binding sequences in their promoters. The senescence process of plants is important for the completion of their life cycle, particularly for crop plants, it is essential for efficient nutrient remobilization during seed filling. It is a highly regulated process, and in order to address the regulatory aspect, the role of genes in the NAC transcription factor family during senescence of barley flag leaves was studied. Several members of the NAC transcription factor gene family were up-regulated during senescence in a microarray experiment, together with a large range of senescence-associated genes, reflecting the coordinated activation of degradation processes in senescing barley leaf tissues. This picture was confirmed in a detailed quantitative reverse transcription–PCR (qRT–PCR) experiment, which also showed distinct gene expression patterns for different members of the NAC gene family, suggesting a group of ~15 out of the 47 studied NAC genes to be important for signalling processes and for the execution of degradation processes during leaf senescence in barley. Seven models for DNA-binding motifs for NAC transcription factors were designed based on published motifs, and available promoter sequences of barley genes were screened for the motifs. Genes up-regulated during senescence showed a significant over-representation of the motifs, suggesting regulation by the NAC transcription factors. Furthermore, co-regulation studies showed that genes possessing the motifs in the promoter in general were highly co-expressed with members of the NAC gene family. In conclusion, a list of up to 15 NAC genes from barley that are strong candidates for being regulatory factors of importance for senescence and biotic stress-related traits affecting the productivity of cereal crop plants has been generated. Furthermore, a list of 71 senescence-associated genes that are potential target genes for these NAC transcription factors is presented.
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DOI：
10.1093/bioinformatics/btr064
发表时间：
2011-04-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Grant CE;Bailey TL;Noble WS
通讯作者：
Noble WS
影响因子：
9.4
作者：
Jukanti, Aravind K.;Heidlebaugh, Nancy M.;Fischer, Andreas M.
通讯作者：
Fischer, Andreas M.
影响因子：
13.8
作者：
Gregersen, Per L.;Holm, Preben Bach
通讯作者：
Holm, Preben Bach
影响因子：
6.4
作者：
LOHMAN, KN;GAN, SS;AMASINO, RM
通讯作者：
AMASINO, RM
影响因子：
56.9
作者：
Kim, Jin Hee;Woo, Hye Ryun;Nam, Hong Gil
通讯作者：
Nam, Hong Gil