Insulin-Like Growth Factor-1 Enhances Motoneuron Survival and Inhibits Neuroinflammation After Spinal Cord Transection in Zebrafish
Insulin-Like Growth Factor-1 Enhances Motoneuron Survival and Inhibits Neuroinflammation After Spinal Cord Transection in Zebrafish
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胰岛素样生长因子-1 可增强斑马鱼脊髓横断后运动神经元的存活并抑制神经炎症
DOI：
10.1007/s10571-020-01022-x
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发表时间：
2021-01
影响因子：
4
通讯作者：
Yan-Qin Shen
中科院分区：
文献类型：
--
作者：
Li-Ping Zhao;Bo-Ping Zhang;Shu-Bing Huang;Zhi-Lan Zhou;Xue-Bing Jia;Chen-Meng Qiao;Fang Wang;Meng-Fei Sun;Yun Shi;Li Yao;Chun Cui;Yan-Qin Shen
胰岛素样生长因子-1（IGF-1）是中枢神经系统局部产生的神经营养因子，可促进轴突再生、保护运动神经元、抑制神经炎症。在本研究中，我们利用斑马鱼脊髓横断模型来探讨IGF-1是否在运动功能恢复中发挥重要作用。与哺乳动物不同，斑马鱼可以在脊髓横断后的极短时间内再生轴突并恢复活动能力。实时定量 PCR 和免疫荧光显示病变部位 IGF-1 表达降低。 IGF-1和Islet-1（运动神经元标记）/GFAP（星形胶质细胞标记）/Iba-1（小胶质细胞标记）双免疫染色显示IGF-1主要表达于运动神经元，周围被星形胶质细胞和小胶质细胞包围。在斑马鱼脊髓横断处的病变部位施用IGF-1吗啉后，游泳试验显示活动性恢复迟缓，运动神经元数量减少，导致小胶质细胞免疫荧光密度增加。我们的数据表明，IGF-1 可增强斑马鱼脊髓横断后运动神经元的存活并抑制神经炎症，这表明 IGF-1 可能参与运动恢复。
Insulin-like growth factor-1 (IGF-1) is a neurotrophic factor produced locally in the central nervous system which can promote axonal regeneration, protect motoneurons, and inhibit neuroinflammation. In this study, we used the zebrafish spinal transection model to investigate whether IGF-1 plays an important role in the recovery of motor function. Unlike mammals, zebrafish can regenerate axons and restore mobility in remarkably short period after spinal cord transection. Quantitative real-time PCR and immunofluorescence showed decreased IGF-1 expression in the lesion site. Double immunostaining for IGF-1 and Islet-1 (motoneuron marker)/GFAP (astrocyte marker)/Iba-1 (microglia marker) showed that IGF-1 was mainly expressed in motoneurons and was surrounded by astrocyte and microglia. Following administration of IGF-1 morpholino at the lesion site of spinal-transected zebrafish, swimming test showed retarded recovery of mobility, the number of motoneurons was reduced, and increased immunofluorescence density of microglia was caused. Our data suggested that IGF-1 enhances motoneuron survival and inhibits neuroinflammation after spinal cord transection in zebrafish, which suggested that IGF-1 might be involved in the motor recovery.
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影响因子：
4.6
作者：
Bollaerts I;Van Houcke J;Andries L;De Groef L;Moons L
通讯作者：
Moons L
影响因子：
4.3
作者：
Nieto-Estévez V;Defterali Ç;Vicario-Abejón C
通讯作者：
Vicario-Abejón C
影响因子：
15.9
作者：
Kreutzberg, GW
通讯作者：
Kreutzberg, GW
影响因子：
2.5
作者：
Shayne E. Boucher;P. Hitchcock
通讯作者：
Shayne E. Boucher;P. Hitchcock
影响因子：
1.5
作者：
Draper, BW;Morcos, PA;Kimmel, CB
通讯作者：
Kimmel, CB