Engineered cartilaginous tubes for tracheal tissue replacement via self-assembly and fusion of human mesenchymal stem cell constructs.
Engineered cartilaginous tubes for tracheal tissue replacement via self-assembly and fusion of human mesenchymal stem cell constructs.
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DOI：
10.1016/j.biomaterials.2015.01.073
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发表时间：
2015-06
期刊：
影响因子：
14
通讯作者：
Alsberg, Eben
中科院分区：
文献类型：
--
作者：
Dikina, Anna D.;Strobel, Hannah A.;Lai, Bradley P.;Rolle, Marsha W.;Alsberg, Eben
关键词：
迫切需要设计一种新气管，因为目前还没有长期治疗影响大部分气道的气管狭窄的方法。在这项工作中，开发了一种模块化气管组织置换策略。通过使用定制设计的培养威尔斯孔和环-管组装系统，成功地产生了高细胞密度、无支架的人间充质干细胞衍生的软骨环和管。此外，将转化生长因子-β1-递送明胶微球掺入工程化组织中，在组织尺寸和基质产生方面以及在环形和管状结构中的分布以及管的管腔刚度方面增强了软骨形成。重要的是，与大鼠气管相比，所有工程组织都具有相似或改善的生物力学特性，这表明它们可以移植到气道缺损的小动物模型中。在本报告中，用于生长基于干细胞的软骨管的模块化、自下而上的方法是一个有前途的平台，可以工程化复杂器官（例如，气管），具有对组织尺寸和几何形状的控制，并且具有用于产生用于人类临床应用的自体组织植入物的潜力。
There is a critical need to engineer a neotrachea because currently there are no long-term treatments for tracheal stenoses affecting large portions of the airway. In this work, a modular tracheal tissue replacement strategy was developed. High-cell density, scaffold-free human mesenchymal stem cell-derived cartilaginous rings and tubes were successfully generated through employment of custom designed culture wells and a ring-to-tube assembly system. Furthermore, incorporation of transforming growth factor-β1-delivering gelatin microspheres into the engineered tissues enhanced chondrogenesis with regard to tissue size and matrix production and distribution in the ring- and tube-shaped constructs, as well as luminal rigidity of the tubes. Importantly, all engineered tissues had similar or improved biomechanical properties compared to rat tracheas, which suggests they could be transplanted in a small animal model for airway defects. The modular, bottom up approach used to grow stem cell-based cartilaginous tubes in this report is a promising platform to engineer complex organs (e.g., trachea), with control over tissue size and geometry, and has the potential to be used to generate autologous tissue implants for human clinical applications.
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影响因子：
4.1
作者：
Livoti, Christine M.;Morgan, Jeffrey R.
通讯作者：
Morgan, Jeffrey R.
影响因子：
4.1
作者：
Berg, Malin;Ejnell, Hasse;Sumitran-Holgersson, Suchitra
通讯作者：
Sumitran-Holgersson, Suchitra
DOI：
10.1016/0304-4165(86)90306-5
发表时间：
1986-09-04
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
FARNDALE, RW;BUTTLE, DJ;BARRETT, AJ
通讯作者：
BARRETT, AJ
影响因子：
4.6
作者：
Fabre, Dominique;Kolb, Frederic;Dartevelle, Philippe
通讯作者：
Dartevelle, Philippe
影响因子：
14
作者：
Huey, Daniel J.;Athanasiou, Kyriacos A.
通讯作者：
Athanasiou, Kyriacos A.