Maturational growth of self-assembled, functional menisci as a result of TGF-β1 and enzymatic chondroitinase-ABC stimulation.
Maturational growth of self-assembled, functional menisci as a result of TGF-β1 and enzymatic chondroitinase-ABC stimulation.
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DOI：
10.1016/j.biomaterials.2010.11.041
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发表时间：
2011-03
期刊：
影响因子：
14
通讯作者：
Athanasiou, Kyriacos A.
中科院分区：
文献类型：
--
作者：
Huey, Daniel J.;Athanasiou, Kyriacos A.
关键词：
置换膝关节半月板的能力需要具有足够几何和生物力学特性的材料。半月板组织工程的尝试已经不能产生具有胶原含量和生物力学性质的组织，特别是拉伸性质，模仿天然半月板。为了获得生化特性重演所需的几何特性和成熟生长，从而获得生物力学特性，将无支架的基于细胞的系统（自组装过程）与分解代谢酶软骨素酶-ABC和TGF-β1结合使用。我们发现，这些试剂的组合导致在成熟的增长，证明了协同增强的径向拉伸模量的5倍和压缩松弛模量的68%，和添加剂的压缩瞬时模量增加136%和Col/WW的196%。这项研究表明，组织工程可以产生一种生物材料，这是等同于天然生物的生化和生物力学性能。
The ability to replace the knee meniscus requires a material possessing adequate geometrical and biomechanical properties. Meniscal tissue engineering attempts have been unable to produce tissue with collagen content and biomechanical properties, particularly tensile properties, mimicking native menisci. In an effort to obtain the geometric properties and the maturational growth necessary for the recapitulation of biochemical and, thus, biomechanical properties, a scaffoldless cell-based system, the self-assembly process, was used in conjunction with the catabolic enzyme chondroitinase-ABC and TGF-β1. We show that combinations of these agents resulted in maturational growth as evidenced by synergistic enhancement of the radial tensile modulus by 5-fold and the compressive relaxation modulus by 68%, and additive increases of the compressive instantaneous modulus by 136% and Col/WW by 196%. This study shows that tissue engineering can produce a biomaterial that is on par with the biochemical and biomechanical properties of native menisci.
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影响因子：
14
作者：
Gunja, Najmuddin J.;Uthamanthil, Rajesh K.;Athanasiou, Kyriacos A.
通讯作者：
Athanasiou, Kyriacos A.
影响因子：
2.8
作者：
SCHMIDT, MB;MOW, VC;EYRE, DR
通讯作者：
EYRE, DR
影响因子：
7
作者：
Imler, SM;Doshi, AN;Levenston, ME
通讯作者：
Levenston, ME
影响因子：
--
作者：
Asanbaeva, Anna;Masuda, Koichi;Sah, Robert L.
通讯作者：
Sah, Robert L.
影响因子：
3.3
作者：
Gunja, Najmuddin J.;Huey, Dan J.;Athanasiou, Kyriacos A.
通讯作者：
Athanasiou, Kyriacos A.