A microarray analysis of sex- and gonad-biased gene expression in the zebrafish: evidence for masculinization of the transcriptome.
A microarray analysis of sex- and gonad-biased gene expression in the zebrafish: evidence for masculinization of the transcriptome.
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DOI：
10.1186/1471-2164-10-579
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发表时间：
2009-12-03
期刊：
影响因子：
4.4
通讯作者：
Jones AG
中科院分区：
文献类型：
--
作者：
Small CM;Carney GE;Mo Q;Vannucci M;Jones AG
在许多分类群中，雄性和雌性在表型上是非常不同的，这些差异往往反映了作用于性别的不同选择压力。表型性二态性几乎肯定反映了性别之间的基因表达的不同模式，微阵列研究已经证明了广泛的性二态性基因表达。虽然基因表达的性别二态性的进化意义仍未得到解决，这些研究已经导致了一个假设，即男性驱动的进化导致了动物转录组的雄性化。在这里，我们使用性别和性腺偏向基因表达的微阵列评估，以测试这一假设在斑马鱼。通过使用斑马鱼的Affyssin微阵列比较基因表达模式在男性和女性的体细胞和性腺组织，我们确定了大量的基因（5899）表现出差异的转录丰度之间的男性和女性的斑马鱼。在保守的统计学显著性标准下，基因表达的所有性别偏差都是由于睾丸和卵巢之间的差异。雄性富集基因比雌性富集基因更丰富，并且雄性富集基因的表达偏好在数量上大于雌性富集基因。我们还鉴定了大量的基因，分别相对于男性身体和女性身体，在睾丸和卵巢中表现出升高的转录丰度。总的来说，我们的研究结果支持这一假设，即男性偏见的进化压力导致了男性偏见的基因表达模式。有趣的是，我们的结果似乎与其他一些基于微阵列的斑马鱼性别特异性基因表达模式的研究不一致。然而，我们的研究是唯一一项旨在解决这一特定假设的研究，研究之间的主要方法差异可以解释这种差异。无论如何，所有这些研究都认为D.尽管明显缺乏异配子体，但rerio分布广泛。这些差异可能对成年斑马鱼的表型性二态性做出重要贡献;因此，从进化的角度来看，性别特异性选择和性冲突在性二态性基因表达进化中的确切作用是非常重要的。我们的研究结果和其他类似的研究结果为进一步的工作奠定了基础，旨在直接解决比较基因组学中这个令人兴奋的问题。
In many taxa, males and females are very distinct phenotypically, and these differences often reflect divergent selective pressures acting on the sexes. Phenotypic sexual dimorphism almost certainly reflects differing patterns of gene expression between the sexes, and microarray studies have documented widespread sexually dimorphic gene expression. Although the evolutionary significance of sexual dimorphism in gene expression remains unresolved, these studies have led to the formulation of a hypothesis that male-driven evolution has resulted in the masculinization of animal transcriptomes. Here we use a microarray assessment of sex- and gonad-biased gene expression to test this hypothesis in zebrafish. By using zebrafish Affymetrix microarrays to compare gene expression patterns in male and female somatic and gonadal tissues, we identified a large number of genes (5899) demonstrating differences in transcript abundance between male and female Danio rerio. Under conservative statistical significance criteria, all sex-biases in gene expression were due to differences between testes and ovaries. Male-enriched genes were more abundant than female-enriched genes, and expression bias for male-enriched genes was greater in magnitude than that for female-enriched genes. We also identified a large number of genes demonstrating elevated transcript abundance in testes and ovaries relative to male body and female body, respectively. Overall our results support the hypothesis that male-biased evolutionary pressures have resulted in male-biased patterns of gene expression. Interestingly, our results seem to be at odds with a handful of other microarray-based studies of sex-specific gene expression patterns in zebrafish. However, ours was the only study designed to address this specific hypothesis, and major methodological differences among studies could explain the discrepancies. Regardless, all of these studies agree that transcriptomic sex differences in D. rerio are widespread despite the apparent absence of heterogamety. These differences likely make important contributions to phenotypic sexual dimorphism in adult zebrafish; thus, from an evolutionary standpoint, the precise roles of sex-specific selection and sexual conflict in the evolution of sexually dimorphic gene expression are very important. The results of our study and others like it set the stage for further work aimed at directly addressing this exciting issue in comparative genomics.
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DOI：
10.1073/pnas.011404098
发表时间：
2001-01-02
影响因子：
11.1
作者：
Li, C;Wong, WH
通讯作者：
Wong, WH
影响因子：
12.3
作者：
Parisi M;Nuttall R;Edwards P;Minor J;Naiman D;Lü J;Doctolero M;Vainer M;Chan C;Malley J;Eastman S;Oliver B
通讯作者：
Oliver B
影响因子：
56.9
作者：
Parisi, M;Nuttall, R;Oliver, B
通讯作者：
Oliver, B
影响因子：
5.8
作者：
Hubbell, E;Liu, WM;Mei, R
通讯作者：
Mei, R
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y