Prolactin acts as a potent survival factor for human breast cancer cell lines.
Prolactin acts as a potent survival factor for human breast cancer cell lines.
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DOI：
10.1038/sj.bjc.6601947
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发表时间：
2004-07-19
影响因子：
8.8
通讯作者：
Holly, JMP
中科院分区：
文献类型：
--
作者：
Perks, CC;Keith, AJ;Goodhew, KL;Savage, PB;Winters, ZE;Holly, JMP
人类乳腺癌是西方社会女性癌症死亡的主要原因，一项大型流行病学研究表明，人类催乳素与乳腺癌风险之间存在密切联系。使用已建立的人乳腺癌细胞系凋亡模型，我们评估了催乳素在乳腺癌细胞生长和存活中的作用。我们发现催乳素对任何细胞系的代谢活性或总细胞数没有影响。我们证实了这些细胞产生内源性催乳素，并且水平不同。在存在催乳素中和抗体的情况下，每种细胞系都响应于诱导细胞凋亡而不是生长抑制。细胞系的敏感性的生理诱导剂的细胞凋亡，C2-神经酰胺，出现相对于内源性催乳素的水平，它们所包含的。然后，我们表明，外源性添加催乳素作为一种有效的生存因子对所有细胞系的细胞凋亡检查。此外，我们证明了催乳素中和抗体与C2-神经酰胺的组合引起了预期的细胞死亡的累加增加。这项研究表明，催乳素保护人类乳腺癌细胞株对凋亡，这可能有重要的意义，癌症治疗。
Human breast cancer is the leading cause of cancer death in women from Western societies, and a large study of the epidemiology demonstrated strong associations between human prolactin and risk of breast cancer. Using established models of apoptosis of human breast cancer cell lines, we assessed the role of prolactin in breast cancer cell growth and survival. We showed that prolactin had no effect on the metabolic activity or total cell number of any cell lines. We confirmed endogenous prolactin production by these cells and that the levels varied. In the presence of a prolactin-neutralising antibody, each of the cell lines responded with the induction of apoptosis as opposed to growth inhibition. The sensitivity of the cell lines to the physiological inducer of apoptosis, C2-ceramide, appeared relative to the levels of endogenous prolactin that they contained. We then showed that exogenously added prolactin acted as a potent survival factor against apoptosis in all the cell lines examined. In addition, we demonstrated that a prolactin-neutralising antibody in combination with C2-ceramide caused an anticipated, additive increase in cell death. This study demonstrated that prolactin protects human breast cancer cell lines against apoptosis and this may have important implications for cancer treatment.
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整联蛋白和神经酰胺信号通路的激活可以抑制人类乳腺癌细胞系中胰岛素样生长因子I（IGF-I）的有丝分裂作用。
影响因子：
8.8
作者：
Perks, C M;Gill, Z P;Newcomb, P V;Holly, J M
通讯作者：
Holly, J M
影响因子：
3.9
作者：
Fernández, ML;Iglesias, MM;Wolfenstein-Todel, C
通讯作者：
Wolfenstein-Todel, C
影响因子：
4
作者：
Peirce, SK;Chen, WY
通讯作者：
Chen, WY
影响因子：
8
作者：
Rose-Hellekant, TA;Arendt, LM;Schuler, LA
通讯作者：
Schuler, LA
影响因子：
4.8
作者：
FUH, G;WELLS, JA
通讯作者：
WELLS, JA