Anthrax toxin receptor 2 is expressed in murine and tumor vasculature and functions in endothelial proliferation and morphogenesis.
Anthrax toxin receptor 2 is expressed in murine and tumor vasculature and functions in endothelial proliferation and morphogenesis.
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DOI：
10.1038/onc.2009.383
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发表时间：
2010-02-11
期刊：
影响因子：
8
通讯作者：
Kitajewski, J.
中科院分区：
文献类型：
--
作者：
Reeves, C. V.;Dufraine, J.;Young, J. A. T.;Kitajewski, J.
毛细形态发生基因2 （CMG2）编码一种炭疽毒素受体（ANTXR2），但其正常生理功能尚不清楚。ANTXR2/CMG2最初是在体外培养的内皮细胞毛细血管形态发生过程中上调的结果。我们探索了血管生成过程的关键步骤依赖于或受ANTXR2/CMG2活性影响的假设。我们描述了ANTXR2/CMG2在几种小鼠组织以及正常乳腺和乳腺肿瘤中的表达模式。在分析的所有组织、培养的内皮细胞和乳腺肿瘤血管中均有内皮表达；然而，ANTXR2/CMG2的表达并不局限于这种细胞类型。为了评估潜在的血管生成功能，我们利用RNA干扰在培养的人脐静脉内皮细胞中显著降低ANTXR2/CMG2的表达。在体外实验中，ANTXR2/CMG2表达降低可显著抑制内皮细胞的增殖，降低内皮细胞形成毛细血管样网络的能力，而在HUVEC中过表达ANTXR2/CMG2可增加内皮细胞的增殖和毛细血管样网络的形成。敲低或过表达对内皮细胞迁移的影响不大。我们得出结论，ANTXR2/CMG2在发芽血管生成过程中具有促进内皮细胞增殖和形态发生的功能，这与ANTXR2/CMG2在几种血管床中的内皮表达一致。
The Capillary Morphogenesis Gene 2 (CMG2) gene encodes an Anthrax toxin receptor (ANTXR2) but the normal physiological function is not known. ANTXR2/CMG2 was originally identified as a result of up-regulation during capillary morphogenesis of endothelial cells cultured in vitro. We explored the hypothesis that key steps of the angiogenic process are either dependent or are influenced by ANTXR2/CMG2 activity. We describe the expression pattern of ANTXR2/CMG2 in several murine tissues and in normal breast and breast tumors. Endothelial expression was found in all of the tissues analyzed, in cultured endothelial cells and in breast tumor vessels; however ANTXR2/CMG2 expression was not restricted to this cell type. To assess potential angiogenic function, we utilized RNA interference to achieve significant reduction of ANTXR2/CMG2 expression in cultured human umbilical venous endothelial cells. Reduced ANTXR2/CMG2 expression resulted in significant inhibition of proliferation and reduced capacity of endothelial cells to form capillary-like networks in vitro, while overexpression of ANTXR2/CMG2 in HUVEC increased proliferation and capillary-like network formation. Little change in migration of endothelial cells was observed upon knockdown or overexpression. We conclude that ANTXR2/CMG2 functions to promote endothelial proliferation and morphogenesis during sprouting angiogenesis, consistent with the endothelial expression of ANTXR2/CMG2 in several vascular beds.
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DOI：
10.1073/pnas.0431098100
发表时间：
2003-04-29
影响因子：
11.1
作者：
Scobie, HM;Rainey, GJA;Young, JAT
通讯作者：
Young, JAT
影响因子：
11.2
作者：
Rogers, Michael S.;Christensen, Kenneth A.;D'Amato, Robert J.
通讯作者：
D'Amato, Robert J.
影响因子：
15.9
作者：
JAFFE, EA;NACHMAN, RL;MINICK, CR
通讯作者：
MINICK, CR
影响因子：
64.8
作者：
Risau, W
通讯作者：
Risau, W
影响因子：
6.4
作者：
Scobie, HM;Thomas, D;Manchester, M
通讯作者：
Manchester, M