Expression of Septin4 in Schistosoma japonicum-infected mouse livers after praziquantel treatment.
Expression of Septin4 in Schistosoma japonicum-infected mouse livers after praziquantel treatment.
复制标题
吡喹酮治疗后日本血吸虫感染小鼠肝脏中Septin4的表达
DOI：
10.1186/s13071-015-0640-9
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发表时间：
2015-01-13
影响因子：
3.2
通讯作者：
Duan Y
中科院分区：
文献类型：
--
作者：
Zhu D;Song K;Chen J;Wang J;Sun X;Qian H;Gu X;Zhang L;Qin Y;Duan Y
背景：Septin 4（SEPT 4）广泛存在于人体组织中，与机械稳定性、肌动蛋白动力学、细胞膜运输、病毒复制和细胞凋亡有关。来自许多研究的数据表明SEPT 4在肝纤维化中起重要作用。SEPT 4在CCl 4和BDL处理的肝纤维化模型中下调。然而，它在感染后12周（p.i.）上调并达到峰值，日本血吸虫（Schistosoma japonicum，S.鸡胚肝纤维化。本研究旨在观察吡喹酮（PZQ）治疗感染沙门氏菌的ICR小鼠后SEPT 4的动态变化。方法：采用Western blot、免疫荧光和qRT-PCR检测SEPT 4的表达。qRT-PCR检测促炎细胞因子IL-6和TNF-α的表达。结果：PZQ抗病毒治疗后，肝星状细胞（HSC）表面SEPT 4的表达明显减少，肉芽肿性炎症减轻，SEPT 4的表达明显降低。SEPT 4的变化趋势与S区的炎症反应呈正相关。结论：PZQ抑制SEPT 4表达可能与减轻慢性和晚期血吸虫虫卵肉芽肿的炎症反应有关。日本血吸虫感染
Background:Septin4 (SEPT4) exists widely in human tissues and is related to mechanical stability, actin dynamics, membrane trafficking, viral replication and apoptosis. Data from many studies have suggested that SEPT4 plays a significant role in liver fibrosis. SEPT4 is down-regulated in the model of CCl4 and BDL treated liver fibrosis. However, it is up-regulated and peaked at 12 weeks post-infection (p.i.), and then decreased subsequently in Schistosoma japonicum (S. japonicum) egg-induced liver fibrosis. The aim of this study was to observe the dynamic alteration of SEPT4 after the treatment of praziquantel (PZQ) in ICR mice infected with S. japonicum.Methods:Expression of SEPT4 was determined by western blot, immunofluorescence and qRT-PCR. And pro-inflammatory cytokines IL-6 and TNF-α were detected by qRT-PCR. The number of eggs, the diameter of egg granulomas and fibrosis-associated genes were also measured.Results:Our results showed that the granulomatous inflammation was reduced, whereafter the expression of SEPT4 on hepatic stellate cells (HSCs) was decreased after PZQ anti-schistosome therapy. And the variation tendency of SEPT4 had positive correlation with the inflammatory response in the area of S. japonicum egg granulomas.Conclusions:Based on these findings, the inhibition of the expression of the SEPT4 by PZQ might be due to alleviation of the inflammatory response at the chronic and advanced stage of S. japonicum infection.
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影响因子：
7.3
作者：
Hams E;Aviello G;Fallon PG
通讯作者：
Fallon PG
影响因子：
4
作者：
Kong X;Horiguchi N;Mori M;Gao B
通讯作者：
Gao B
影响因子：
64.8
作者：
AMIRI, P;LOCKSLEY, RM;MCKERROW, JH
通讯作者：
MCKERROW, JH
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
Iwaisako, Keiko;Hatano, Etsuro;Kinoshita, Makoto
通讯作者：
Kinoshita, Makoto