Development of an on-chip injector for microchip-based flow analyses using laminar flow.
Development of an on-chip injector for microchip-based flow analyses using laminar flow.
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开发用于使用层流进行基于微芯片的流动分析的片上注射器。
DOI：
10.1039/b707410g
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发表时间：
2007
期刊：
影响因子：
6.1
通讯作者：
Martin,RScott
中科院分区：
文献类型：
--
作者：
Moehlenbrock,MichaelJ;Martin,RScott
设计并表征了一种用于基于微芯片的流量分析的新型片上注射器。微芯片设计利用缓冲液和样品的独立层流，它们平行接触的距离为 300 µm。缓冲液流首先通过装有 5 µm 内径的传统 6 端口进样阀。样本循环。当 6 通阀在给定时间内从负载启动到注射时，片上缓冲液流会收缩，并且样品流会被加压进入缓冲液流通道。一旦阀门返回到负载状态，单独的层流就会恢复。使用荧光检测来表征进样器，发现 100 µM 荧光素样品进样 50 次后，平均峰高为 174.32 ± 2.05 AFU (RSD 1.18%)，平均峰偏度为 1.37 ± 0.06。注射器还与电流检测连接。注射浓度范围为 500 nM 至 100 µM 的儿茶酚溶液会产生线性响应（灵敏度 = 2.49 pA µM−1，r2 = 0.998），检测限为 155 nM (S/N = 3)。与片外注入方案相比，塞稀释、谱带展宽和峰不对称性大大减少。最后，演示了红细胞样品的注射和随后的裂解，注射的红细胞塞在注射到含有十二烷基硫酸钠的液流中后 5.72 ± 0.15 秒裂解（n = 10）。新的注射方案不需要复杂的阀门机构或高压，并且能够以高时间分辨率监测分析物浓度变化的方式从连续的样品流中进行可重复的注射。
A new on-chip injector for microchip-based flow analyses has been designed and characterized. The microchip design utilizes separate laminar flow streams of buffer and sample that are brought into parallel contact for a distance of 300 µm. The buffer flow stream is first routed through a conventional 6-port injection valve fitted with a 5 µm i.d. sample loop. When the 6-port valve is actuated from load to inject for a given time, the on-chip buffer flow stream is constricted and the sample flow stream is pressurized into the buffer flow channel. Once the valve returns to the load state the separate laminar flow streams resume. Fluorescence detection was used to characterize the injector and it was found that 50 injections of a 100 µM fluorescein sample led to an average peak height of 174.32 ± 2.05 AFU (RSD 1.18%) and average peak skew of 1.37 ± 0.06. The injector was also interfaced with amperometric detection. Injections of catechol solutions ranging in concentration from 500 nM to 100 µM resulted in a linear response (sensitivity = 2.49 pA µM−1, r2 = 0.998) and a limit of detection of 155 nM (S/N = 3). Compared to an off-chip injection scheme, plug dilution, band broadening, and peak asymmetry are much reduced. Finally, the injection and subsequent lysis of an erythrocyte sample was demonstrated, with an injected plug of erythrocytes being lysed 5.72 ± 0.15 s after injection into a flow stream containing sodium dodecyl sulfate (n = 10). The new injection scheme does not require complex valving mechanisms or high pressures and enables reproducible injections from a continuous sample flow stream in a manner where changes in analyte concentration can be monitored with high temporal resolution.
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