Extracellular ATP elicits DORN1-mediated RBOHD phosphorylation to regulate stomatal aperture.
Extracellular ATP elicits DORN1-mediated RBOHD phosphorylation to regulate stomatal aperture.
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DOI：
10.1038/s41467-017-02340-3
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发表时间：
2017-12-22
影响因子：
16.6
通讯作者：
Stacey G
中科院分区：
文献类型：
--
作者：
Chen D;Cao Y;Li H;Kim D;Ahsan N;Thelen J;Stacey G
除了作为细胞能量来源外，ATP还可以作为动物和植物中的损伤相关分子模式。气孔是控制气体交换的气孔，因此影响光合作用、耐旱性等关键功能，也是病原体的首选进入点。在这里，我们表明，ATP的加入导致叶片气孔的快速关闭，并增强了对细菌病原体Psuedomonasseringae的抗性。这种反应是由受体DORN 1识别ATP介导的，随后是NADPH氧化酶RBOHD的直接磷酸化，导致活性氧的产生增加和气孔关闭。RBOHD上DORN 1磷酸化位点的突变消除了ATP诱导气孔关闭的能力。这些数据暗示了通过DORN 1控制气孔孔径的嘌呤能信号传导对控制植物光合作用、水稳态、病原体抗性和最终产量具有重要意义。细胞外ATP作为一种损伤相关的分子模式，触发植物对创伤和环境刺激的信号反应。Chen等人在这里表明，DORN 1对ATP的感知可以通过RBOHD磷酸化和ROS产生来触发气孔关闭。
In addition to acting as a cellular energy source, ATP can also act as a damage-associated molecular pattern in both animals and plants. Stomata are leaf pores that control gas exchange and, therefore, impact critical functions such as photosynthesis, drought tolerance, and also are the preferred entry point for pathogens. Here we show the addition of ATP leads to the rapid closure of leaf stomata and enhanced resistance to the bacterial pathogen Psuedomonas syringae. This response is mediated by ATP recognition by the receptor DORN1, followed by direct phosphorylation of the NADPH oxidase RBOHD, resulting in elevated production of reactive oxygen species and stomatal closure. Mutation of DORN1 phosphorylation sites on RBOHD eliminates the ability of ATP to induce stomatal closure. The data implicate purinergic signaling via DORN1 in the control of stomatal aperture with important implications for the control of plant photosynthesis, water homeostasis, pathogen resistance, and ultimately yield. Extracellular ATP acts as a damage-associated molecular pattern that triggers signaling responses to wounding and environmental stimuli in plants. Here Chen et al. show that ATP perception by DORN1 can trigger stomatal closure mediated via RBOHD phosphorylation and ROS production.
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影响因子：
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