Effects of Pregnancy on Plasma Sphingolipids Using a Metabolomic and Quantitative Analysis Approach.
Effects of Pregnancy on Plasma Sphingolipids Using a Metabolomic and Quantitative Analysis Approach.
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使用代谢组学和定量分析方法，妊娠对血浆鞘脂的影响。
DOI：
10.3390/metabo13091026
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发表时间：
2023-09-21
期刊：
影响因子：
4.1
通讯作者：
Hebert MF
中科院分区：
文献类型：
--
作者：
Enthoven LF;Shi Y;Fay E;Kim A;Moreni S;Mao J;Isoherranen N;Totah RA;Hebert MF
在怀孕期间发生的母体代谢组的变化，特别是母体脂体的变化相对未知。这项研究的目的是利用代谢组学分析，然后进行确证的、有针对性的定量分析，来评估怀孕对鞘磷脂水平的影响。我们专注于鞘磷脂的三个亚类：神经酰胺、鞘磷脂和鞘磷脂。47名年龄在18岁到50岁之间的孕妇参与了这项研究。在两个研究日采集血样进行代谢组学分析。孕周为25~28周，产后研究日为产后3个月≥。每个参与者都充当自己的对照。这些样品用超高效液/质/质(UPLC/MS/MS)分析，产生半定量的峰面积值，用于比较怀孕和产后的鞘磷脂水平。在此脂组学分析之后，对相同的研究样本进行了定量LC/MS/MS靶向/验证性分析。在代谢组学分析中，鉴定了43种鞘磷脂代谢物，并用相对峰面积值评估了它们的水平。这些神经鞘磷脂分为三类：神经酰胺、神经鞘磷脂和神经鞘糖脂。在测量的43种分析物中，35种在妊娠期有显著差异(p<；0.05)(包括7种神经酰胺、26种鞘磷脂和2种鞘氨醇)，32种在妊娠期显著高于产后。在代谢组学之后，进行了单独的定量分析，得到了23种不同鞘磷脂的量化浓度值，其中4种也在代谢组学研究中被检测到。定量分析支持代谢组学结果，23种分析物中有17种在怀孕期间发现显著不同，包括11种神经酰胺、4种鞘磷脂和2种鞘氨醇。其中14个在怀孕期间显著升高。我们的数据表明，血浆鞘脂浓度总体上增加，可能与内皮功能、妊娠期糖尿病(GDM)、妊娠期肝内胆汁淤积症和胎儿发育有关。这项研究为怀孕期间母体鞘脂代谢的变化提供了证据。
Changes in the maternal metabolome, and specifically the maternal lipidome, that occur during pregnancy are relatively unknown. The objective of this investigation was to evaluate the effects of pregnancy on sphingolipid levels using metabolomics analysis followed by confirmational, targeted quantitative analysis. We focused on three subclasses of sphingolipids: ceramides, sphingomyelins, and sphingosines. Forty-seven pregnant women aged 18 to 50 years old participated in this study. Blood samples were collected on two study days for metabolomics analysis. The pregnancy samples were collected between 25 and 28 weeks of gestation and the postpartum study day samples were collected ≥3 months postpartum. Each participant served as their own control. These samples were analyzed using a Ultra-performance liquid chromatography/mass spectroscopy/mass spectroscopy (UPLC/MS/MS) assay that yielded semi-quantitative peak area values that were used to compare sphingolipid levels between pregnancy and postpartum. Following this lipidomic analysis, quantitative LC/MS/MS targeted/confirmatory analysis was performed on the same study samples. In the metabolomic analysis, 43 sphingolipid metabolites were identified and their levels were assessed using relative peak area values. These profiled sphingolipids fell into three categories: ceramides, sphingomyelins, and sphingosines. Of the 43 analytes measured, 35 were significantly different during pregnancy (p < 0.05) (including seven ceramides, 26 sphingomyelins, and two sphingosines) and 32 were significantly higher during pregnancy compared to postpartum. Following metabolomics, a separate quantitative analysis was performed and yielded quantified concentration values for 23 different sphingolipids, four of which were also detected in the metabolomics study. Quantitative analysis supported the metabolomics results with 17 of the 23 analytes measured found to be significantly different during pregnancy including 11 ceramides, four sphingomyelins, and two sphingosines. Fourteen of these were significantly higher during pregnancy. Our data suggest an overall increase in plasma sphingolipid concentrations with possible implications in endothelial function, gestational diabetes mellitus (GDM), intrahepatic cholestasis of pregnancy, and fetal development. This study provides evidence for alterations in maternal sphingolipid metabolism during pregnancy.
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影响因子：
3.7
作者：
Charkiewicz K;Goscik J;Blachnio-Zabielska A;Raba G;Sakowicz A;Kalinka J;Chabowski A;Laudanski P
通讯作者：
Laudanski P
影响因子：
4.1
作者：
通讯作者：
--
DOI：
10.1111/j.1471-0528.2008.01906.x
发表时间：
2009-01-01
影响因子：
5.8
作者：
Khalil, A. A.;Cooper, D. J.;Harrington, K. F.
通讯作者：
Harrington, K. F.
影响因子：
0.7
作者：
Soma-Pillay P;Nelson-Piercy C;Tolppanen H;Mebazaa A
通讯作者：
Mebazaa A
影响因子：
3.7
作者：
Spijkers LJ;van den Akker RF;Janssen BJ;Debets JJ;De Mey JG;Stroes ES;van den Born BJ;Wijesinghe DS;Chalfant CE;MacAleese L;Eijkel GB;Heeren RM;Alewijnse AE;Peters SL
通讯作者：
Peters SL