RNAi-mediated disruption of squalene synthase improves drought tolerance and yield in rice.
RNAi-mediated disruption of squalene synthase improves drought tolerance and yield in rice.
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DOI：
10.1093/jxb/err258
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发表时间：
2012-01
影响因子：
6.9
通讯作者：
Nguyen HT
中科院分区：
文献类型：
--
作者：
Manavalan LP;Chen X;Clarke J;Salmeron J;Nguyen HT
世界上大约三分之一的水稻种植面积在雨水灌溉的低地，大多数都容易缺水。在模式谷类植物水稻中鉴定赋予耐旱性的基因是一种有吸引力的策略，不仅可以工程化改良水稻的耐旱性，还可以工程化改良其它谷类的耐旱性。研究表明，玉米角鲨烯合酶对水稻法尼基转移酶/角鲨烯合酶（SQS）的RNAi介导的破坏提高了营养和生殖阶段的耐旱性。野生型（日本晴）的20天龄幼苗和转基因RNAi株系的7个独立事件在形态上没有表现出差异。当在生长室条件下经受32 d的水分胁迫时，转基因阳性植株表现出延迟萎蔫、保存更多的土壤水分和改善恢复。当5个独立事件沿着野生型植物在温室条件下的生殖阶段遭受干旱时，转基因植物与野生型相比，通过降低气孔导度和保持高的叶片相对含水量（RWC），水分损失更慢。经过28天的缓慢渐进的土壤干燥，转基因植物恢复得更好，开花早于野生型植物。水分胁迫转基因阳性植物的产量比野生型植物高14-39%。当在含有1.2%琼脂的Yoshida营养液的平板中生长时，来自三个独立事件的转基因阳性显示出根长度增加和侧根数量增加。RNAi介导的失活导致气孔导度降低，随后产生耐旱性。
About one-third of the world’s rice area is in rain-fed lowlands and most are prone to water shortage. The identification of genes imparting tolerance to drought in the model cereal plant, rice, is an attractive strategy to engineer improved drought tolerance not only rice but other cereals as well. It is demonstrated that RNAi-mediated disruption of a rice farnesyltransferase/squalene synthase (SQS) by maize squalene synthase improves drought tolerance at both the vegetative and reproductive stages. Twenty-day-old seedlings of wild type (Nipponbare) and seven independent events of transgenic RNAi lines showed no difference in morphology. When subjected to water stress for a period of 32 d under growth chamber conditions, transgenic positives showed delayed wilting, conserved more soil water, and improved recovery. When five independent events along with wild-type plants were subjected to drought at the reproductive stage under greenhouse conditions, the transgenic plants lost water more slowly compared with the wild type, through reduced stomatal conductance and the retention of high leaf relative water content (RWC). After 28 d of slow progressive soil drying, transgenic plants recovered better and flowered earlier than wild-type plants. The yield of water-stressed transgenic positive plants ranged from 14–39% higher than wild-type plants. When grown in plates with Yoshida’s nutrient solution with 1.2% agar, transgenic positives from three independent events showed increased root length and an enhanced number of lateral roots. The RNAi-mediated inactivation produced reduced stomatal conductance and subsequent drought tolerance.
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影响因子：
56.9
作者：
Cutler, S;Ghassemian, M;McCourt, P
通讯作者：
McCourt, P
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.
DOI：
10.1073/pnas.0604882103
发表时间：
2006-08-29
影响因子：
11.1
作者：
Hu, Honghong;Dai, Mingqiu;Xiong, Lizhong
通讯作者：
Xiong, Lizhong
DOI：
10.1073/pnas.0707294104
发表时间：
2007-09-25
影响因子：
11.1
作者：
Karaba, Aarati;Dixit, Shital;Pereira, Andy
通讯作者：
Pereira, Andy
影响因子：
3.8
作者：
HAUDENSCHILD, C;HARTMANN, MA
通讯作者：
HARTMANN, MA