Th1/Th2 cytokine ratio in tissue transudates from patients with oral lichen planus.
Th1/Th2 cytokine ratio in tissue transudates from patients with oral lichen planus.
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DOI：
10.1155/2007/19854
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发表时间：
2007
影响因子：
4.6
通讯作者：
Ondrey F
中科院分区：
文献类型：
--
作者：
Rhodus NL;Cheng B;Ondrey F
客观的。口腔扁平苔藓（OLP）的特征促使研究人员探索可能的生物标志物，通过这些生物标志物来监测疾病活动性和治疗效果。口腔液可以为此类生物标志物的分析提供可利用的介质。先前的研究表明，核因子κB（NF-κB）的激活在口腔扁平苔藓（OLP）的发病机制中发挥着重要作用，OLP是一种由T细胞介导的慢性炎症性疾病。在本次研究之前，尚未有关于口腔液中 NF-κ B 及其依赖性细胞因子水平的报告。本研究的目的是检测OLP病灶直接组织渗出液中NF-κB依赖性细胞因子、TNF-α、IL-1-α、IL-6和IL-8的水平，并探讨数据应用于临床的可行性。学习设计。 13 名确诊的 OLP 受试者以及 13 名年龄性别匹配的对照者参加了该研究。在每个受试者中，通过一种新颖的滤纸收集技术收集病变组织渗出液（TT）。采用 ELISA 法测定三种口腔液中细胞因子 TNF-α、IL-1-α、IL-6 和 IL-8 的水平。结果。在组织漏出液（TT）中，OLP患者检测到的细胞因子TNF-α、IL-1-α、IL-6和IL-8水平显着高于对照组：（TT：40.0±9.8 vs 4.5±0.7、710±114 vs 305±78、150±25 vs 1.7±0.5、2800± 260 与 1450 ± 130，P < .0001；单位：pg/mL）。结论。这些结果表明，口腔病变组织渗出液中可能检测到 NF-κ B 依赖性炎症细胞因子水平升高，这可能具有监测 OLP 患者疾病活动性和做出治疗决策的诊断和预后潜力。
Objective. The characteristics of oral lichen planus (OLP) provoke investigators to explore possible biomarkers by which to monitor disease activity and therapeutic efficacy. Oral fluids may provide an accessible medium for analysis of such biomarkers. Previous studies have shown that activation of nuclear factor-kappa B (NF-κ B) plays an important role in the pathogenesis of oral lichen planus (OLP), which is a chronic inflammatory disorder mediated by T cells. Prior to the present investigation, reports of the levels of NF-κ B and its dependent cytokines in oral fluids have not been forthcoming. The purpose of this study was to detect the level of NF-κ B dependent cytokines, TNF-alpha, IL-1-alpha, IL-6, and IL-8 in tissue transudates directly from lesions of OLP, and explore the feasibility of the data for clinical application. Study design. Thirteen definitively diagnosed OLP subjects were enrolled in the study as were 13 age-sex matched controls. In each subject, lesion tissue transudates (TTs) were collected by a novel collection technique with a filter paper. The level of cytokines, TNF-alpha, IL-1-alpha, IL-6, and IL-8 in three types of oral fluids were determined by ELISA. Results. In the tissue transudate(TT), there were significantly higher level of cytokines TNF-alpha, IL-1-alpha, IL-6, and IL-8 detected in OLP patients than in controls: (TT: 40.0 ± 9.8 versus 4.5 ± 0.7, 710 ± 114 versus 305 ± 78, 150 ± 25 versus 1.7 ± 0.5, 2800 ± 260 versus 1450 ± 130, P < .0001; unit: pg/mL). Conclusions. These results indicate that NF-κ B dependent inflammatory cytokines may be detected at increased levels in oral lesion tissue transudates which may have diagnostic and prognostic potentials for monitoring disease activity and making therapeutic decisions in patients with OLP.
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影响因子：
4.6
作者：
Pezelj-Ribaric, Sonja;Prso, Ivana Brekalo;Abram, Maja;Glazar, Irena;Brumini, Gordana;Simunovic-Soskic, Marica
通讯作者：
Simunovic-Soskic, Marica
影响因子：
3.6
作者：
Diehl, S;Rincón, M
通讯作者：
Rincón, M
影响因子：
82.9
作者：
Ghoreschi, K;Thomas, P;Röcken, M
通讯作者：
Röcken, M
影响因子：
6.5
作者：
Karatsaidis, A;Schreurs, O;Schenck, K
通讯作者：
Schenck, K
影响因子：
3.3
作者：
Khan, A;Farah, CS;Sugerman, PB
通讯作者：
Sugerman, PB