Cloning and characterization of the actin gene from Puccinia striiformis f. sp. tritici
Cloning and characterization of the actin gene from Puccinia striiformis f. sp. tritici
复制标题
条锈菌肌动蛋白基因的克隆和表征。
DOI：
10.1007/s11274-012-1040-3
复制
发表时间：
2012-03
影响因子：
4.1
通讯作者：
Zhensheng Kang
中科院分区：
文献类型：
--
作者：
Jie Liu;Qiong Zhang;Qing Chang;Hua Zhuang;Lili Huang;Zhensheng Kang
小麦条锈病的病原小麦条锈病菌是专性生物营养担子菌。脲孢子是本病流行病学中最常见的孢子类型。小麦芽孢萌发芽管尖部生长是小麦侵染的关键步骤，但迄今为止对其研究较少。最近的研究发现肌动蛋白与菌丝尖端的生长密切相关。在本研究中，我们克隆并获得了小麦纹状芽孢杆菌肌动蛋白全长cDNA，并对其表达进行了表征。此外，在试管形成过程中，在显微镜下可以看到肌动蛋白丝（F-actin）的模式。最明显的含肌动蛋白结构是肌动蛋白斑块。它们主要集中在菌丝尖端附近，分散在整个皮层。利用细胞松弛素B (cytochalasin B)，我们观察到F-actin的解聚大大降低了尿素孢子的发芽率，并破坏了囊泡到芽管尖端的运输，这表明F-actin在小麦条纹状芽管尖端生长中起着关键作用。本研究有助于我们了解小麦纹状芽孢杆菌的尖端生长机制，并为设计新型农药提供理论框架。
The fungus Puccinia striiformis f. sp. tritici, the causal agent of wheat stripe rust, is an obligate biotrophic basidiomycete. Urediniospores are the most common spore type involved in the epidemiology of this disease. Tip growth of germ tubes of germinated urediniospores is a key step during infection of wheat, but few studies have investigated it so far. Recent research has found that actin is closely associated with hyphal tip growth. In this study, we have cloned and obtained the full-length actin cDNA from P. striiformis f. sp. tritici and characterized its expression. Furthermore, actin filament (F-actin) patterns were visualized microscopically during germ tube formation. The most conspicuous actin-containing structures were actin patches. They were mainly concentrated near the hyphal tip and scattered throughout the cortex. By using cytochalasin B, we observed that depolymerization of F-actin greatly reduced the germination rate of urediniospores and disrupted the transport of vesicles to the germ tube tip, indicating that F-actin played a key role in the tip growth of P. striiformis f. sp. tritici. This work helps us to understand the tip growth mechanism of P. striiformis f. sp. tritici, and may provide a theoretical framework for designing novel pesticides.
登录
查看更多内容
影响因子：
2.1
作者：
F. Barja;B. N. Thi;G. Turian
通讯作者：
F. Barja;B. N. Thi;G. Turian
DOI：
--
发表时间：
2007
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
H. Hassouni;M. Alaoui;K. Lamrani;I. Perraud;C. Augur;S. Roussos
通讯作者：
H. Hassouni;M. Alaoui;K. Lamrani;I. Perraud;C. Augur;S. Roussos
影响因子：
5.3
作者：
K. Hirayoshi;H. Kudo;H. Takechi;A. Nakai;A. Iwamatsu;K. Yamada;K. Nagata
通讯作者：
K. Hirayoshi;H. Kudo;H. Takechi;A. Nakai;A. Iwamatsu;K. Yamada;K. Nagata
DOI：
--
发表时间：
2003
期刊：
--
影响因子：
--
作者：
Z. Kang;Lili Huang;H. Buchenauer
通讯作者：
Z. Kang;Lili Huang;H. Buchenauer
影响因子：
--
作者：
Aleksandra Virag;Steven D Harris
通讯作者：
Aleksandra Virag;Steven D Harris