Transcriptome analysis of human adipocytes implicates the NOD-like receptor pathway in obesity-induced adipose inflammation.
Transcriptome analysis of human adipocytes implicates the NOD-like receptor pathway in obesity-induced adipose inflammation.
复制标题
DOI：
10.1016/j.mce.2014.06.018
复制
发表时间：
2014-08-25
影响因子：
4.1
通讯作者：
Hsueh WA
中科院分区：
文献类型：
--
作者：
Yin Z;Deng T;Peterson LE;Yu R;Lin J;Hamilton DJ;Reardon PR;Sherman V;Winnier GE;Zhan M;Lyon CJ;Wong ST;Hsueh WA
脂肪组织炎症随着肥胖而增加，但脂肪细胞与免疫细胞的贡献尚不清楚。在本研究中，转录组分析进行了高度纯化的皮下脂肪细胞从瘦和肥胖的妇女，差异表达的基因/途径，确定在脂肪细胞和基质血管部分（SVF）样品。NOD样受体途径基因（包括NLRP 3和PYCARD）的脂肪细胞表达（而非SVF表达）与肥胖表型和脂肪细胞II类主要组织相容性复合物（MHCII）基因表达相关，其中NLRP 3和PYCARD调节caspase-1介导的IL-1β分泌，但在调整年龄和体重指数后，仅MHCII保持不变。IFNγ刺激脂肪细胞MHCII、NLRP 3和caspase-1的表达，而脂肪细胞MHCII介导的CD 4 + T细胞活化（脂肪炎症的重要因素）诱导IFNγ依赖性脂肪细胞IL-1β分泌。这些结果揭示了由T细胞IFNγ和脂肪细胞MHCII和NLRP 3炎性体活性之间的相互作用调节的对话，其似乎在肥胖期间启动和升级脂肪组织炎症。
Adipose tissue inflammation increases with obesity, but adipocyte vs. immune cell contributions are unclear. In the present study, transcriptome analyses were performed on highly-purified subcutaneous adipocytes from lean and obese women, and differentially expressed genes/pathways were determined in both adipocyte and stromal vascular fraction (SVF) samples. Adipocyte but not SVF expression of NOD-like receptor pathway genes, including NLRP3 and PYCARD, which regulate caspase-1-mediated IL-1β secretion, correlated with adiposity phenotypes and adipocyte class II major histocompatibility complex (MHCII) gene expression, but only MHCII remained after adjusting for age and body mass index. IFNγ stimulated adipocyte MHCII, NLRP3 and caspase-1 expression, while adipocyte MHCII-mediated CD4+ T cell activation, an important factor in adipose inflammation, induced IFNγ-dependent adipocyte IL-1β secretion. These results uncover a dialogue regulated by interactions among T cell IFNγ and adipocyte MHCII and NLRP3 inflammasome activity that appears to initiate and escalate adipose tissue inflammation during obesity.
登录
查看更多内容
影响因子：
29
作者：
Deng T;Lyon CJ;Minze LJ;Lin J;Zou J;Liu JZ;Ren Y;Yin Z;Hamilton DJ;Reardon PR;Sherman V;Wang HY;Phillips KJ;Webb P;Wong ST;Wang RF;Hsueh WA
通讯作者：
Hsueh WA
影响因子：
6.9
作者：
Bergman, Richard N.;Stefanovski, Darko;Buchanan, Thomas A.;Sumner, Anne E.;Reynolds, James C.;Sebring, Nancy G.;Xiang, Anny H.;Watanabe, Richard M.
通讯作者：
Watanabe, Richard M.
DOI：
10.4049/jimmunol.0802367
发表时间：
2009-03-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Bryan NB;Dorfleutner A;Rojanasakul Y;Stehlik C
通讯作者：
Stehlik C
影响因子：
30.5
作者：
通讯作者：
--
影响因子：
5.7
作者：
Moschen, Alexander R.;Molnar, Clemens;Tilg, Herbert
通讯作者：
Tilg, Herbert