Gadolinium blocks membrane permeabilization induced by nanosecond electric pulses and reduces cell death.
Gadolinium blocks membrane permeabilization induced by nanosecond electric pulses and reduces cell death.
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DOI：
10.1016/j.bioelechem.2009.12.007
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发表时间：
2010-08
影响因子：
5
通讯作者：
Pakhomov, Andrei G.
中科院分区：
文献类型：
--
作者：
Andre, Franck M.;Rassokhin, Mikhail A.;Bowman, Angela M.;Pakhomov, Andrei G.
纳秒电脉冲（nsEP）与微秒和毫秒持续时间脉冲的区别在于其引起细胞内效应和细胞死亡的能力，并且对细胞质膜的影响降低，这已被广泛接受。然而，我们发现，nsEP诱导的细胞死亡是最有可能介导的质膜破坏。我们发现，nsEP可以引起质膜电导的长期（分钟）增加，并破坏电解质平衡，随后是水分吸收，细胞肿胀和起泡。这些质膜透化的影响可以被Gd 3+以剂量依赖性方式阻断，其阈值在亚微摩尔浓度。因此，Gd 3+保护细胞免于nsEP诱导的细胞死亡，从而指出质膜透化是致死性细胞损伤的可能的主要机制。
It has been widely accepted that nanosecond electric pulses (nsEP) are distinguished from micro-and millisecond duration pulses by their ability to cause intracellular effects and cell death with reduced effects on the cell plasma membrane. However, we found that nsEP-induced cell death is most likely mediated by the plasma membrane disruption. We showed that nsEP can cause long-lasting (minutes) increase in plasma membrane electrical conductance and disrupt electrolyte balance, followed by water uptake, cell swelling and blebbing. These effects of plasma membrane permeabilization could be blocked by Gd3+ in a dose-dependent manner, with a threshold at sub-micromolar concentrations. Consequently, Gd3+ protected cells from nsEP-induced cell death, thereby pointing to plasma membrane permeabilization as a likely primary mechanism of lethal cell damage.
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影响因子：
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