Tissue-specific expression of bone proteins in femora of growing rats.
Tissue-specific expression of bone proteins in femora of growing rats.
复制标题
生长大鼠股骨中骨蛋白的组织特异性表达。
DOI：
10.1152/ajpendo.1992.263.4.e724
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Spelsberg,TC
中科院分区：
文献类型：
--
作者：
Turner,RT;Kapelner,SN;Spelsberg,TC
从2月龄大鼠三种不同股骨室的骨细胞中提取总细胞RNA。首先将完整股骨与胶原酶孵育以获得骨膜细胞。然后将等分的无骨膜骨干和干骺端与胶原酶一起孵育，以分别获得骨内膜和松质骨细胞的富集群体。使用琼脂糖凝胶电泳按大小分离来自这三种组织的总细胞RNA，转移到尼龙过滤器上，与32 P标记的甘油醛-3-磷酸脱氢酶（GAP）、前α（I）原I型胶原（胶原）、骨钙素（BGP）和碱性磷酸酶（AP）的cDNA探针杂交，并通过放射自显影术观察cDNA/mRNA杂交体。通过基于四环素的动态骨组织形态计量学测量每个组织隔室中的骨基质沉积。骨形成和沉积率以骨膜最高，干骺端最低。胶原和BGP的平均mRNA水平与平均骨形成和矿物质沉积率呈正相关。有趣的是，平均AP mRNA水平与骨形成指数无关。这些结果表明，在股骨骨基质蛋白的稳态mRNA水平显示出明显的位点特异性，并与骨基质沉积率相关。
Total cellular RNA was extracted from bone cells of three different femoral compartments of 2-mo-old rats. The intact femora were first incubated with collagenase to obtain periosteal cells. The bisected periosteum-free diaphyses and metaphyses were then incubated with collagenase to obtain enriched populations of endosteal and cancellous bone cells, respectively. The total cellular RNA from these three tissues was separated by size using agarose gel electrophoresis, transferred to nylon filters, hybridized to 32P-labeled cDNA probes for glyceraldehyde-3-phosphate dehydrogenase (GAP), pre-pro-alpha (I) type I collagen (collagen), osteocalcin (BGP), and alkaline phosphatase (AP), and the cDNA/mRNA hybrids were visualized by radioautography. Bone matrix deposition was measured in each tissue compartment by tetracycline-based dynamic bone histomorphometry. The bone formation and apposition rates were greatest in the periosteum and least in metaphysis. Mean mRNA levels for collagen and BGP were positively correlated with mean bone formation and mineral apposition rates. Interestingly, mean AP mRNA levels were not correlated with indexes of bone formation. These results demonstrate that the steady-state mRNA levels for bone matrix proteins in femora show pronounced site specificity and correlate with the rates of bone matrix deposition.
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DOI：
10.1073/pnas.85.6.1796
发表时间：
1988-03
影响因子：
11.1
作者：
K. Fink;E. Wieben;G. Woloschak;T. Spelsberg
通讯作者：
K. Fink;E. Wieben;G. Woloschak;T. Spelsberg
真核生物中蛋白质合成起始的调节。
影响因子：
3.9
作者：
S. Ochoa
通讯作者：
S. Ochoa
离体大鼠胫骨骨膜细胞未能将 5α 睾酮还原为 5α-二氢睾酮
DOI：
--
发表时间：
1990
期刊：
影响因子：
--
作者：
R. Turner;B. Bleiberg;D. Colvard;P. Keeting;G. Evans;T. Spelsberg
通讯作者：
T. Spelsberg
三种骨硬化大鼠突变骨骼发育过程中的基因表达。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Shalhoub,V;Jackson,ME;Lian,JB;Stein,GS;MarksJr,SC
通讯作者：
MarksJr,SC