Wheat-ghretropins: novel ghrelin-releasing peptides derived from wheat protein.
Wheat-ghretropins: novel ghrelin-releasing peptides derived from wheat protein.
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DOI：
10.1002/2211-5463.13124
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发表时间：
2021-04
期刊：
影响因子：
2.6
通讯作者：
Ohinata K
中科院分区：
文献类型：
--
作者：
Tanikawa K;Kaneko K;Abe S;Nakato J;Tokuyama Y;Odaka S;Iwakura H;Sato M;Kurabayashi A;Suzuki H;Makita M;Ikemoto H;Matsumura S;Inoue K;Ohinata K
Ghrelin是一种主要由胃细胞产生的内源性促食欲激素，据报道其影响食欲、胃肠运动和生长激素分泌。我们观察到，酶消化小麦面筋刺激生长激素释放肽释放细胞系小鼠生长激素瘤3 - 1的生长激素释放肽分泌。此外，我们通过使用液相色谱-质谱法和结构-活性关系的综合肽分析来表征消化物中存在的生长素释放肽。在候选肽中，我们发现SQQQQPVLPQQPSF，LSVTSPQQVSY和YPTSL刺激ghrelin释放。然后，我们分别将它们命名为小麦促生长素A、B和C。此外，我们观察到小麦生长激素释放肽A在口服给药后增加了小鼠的血浆生长激素释放肽浓度和食物摄入量。因此，我们证明了小麦促生长素释放肽在体外和体内都能刺激生长素释放。据我们所知，这是第一份关于小麦来源的外源性生物活性肽刺激ghrelin分泌的报告。通过对谷蛋白消化物进行肽综合分析，并与已知的生长素释放肽进行肽同源性比较，我们发现了三种新的肽，我们将其命名为小麦促生长素A、B和C。我们观察到，小麦生长素A在小鼠口服给药后增加了食物摄入量和血清生长素释放肽水平。据我们所知，这是第一份关于小麦来源的外源性生物活性肽刺激ghrelin分泌的报告。
Ghrelin is an endogenous orexigenic hormone mainly produced by stomach cells and is reported to influence appetite, gastrointestinal motility and growth hormone secretion. We observed that enzymatic digest of wheat gluten stimulated ghrelin secretion from mouse ghrelinoma 3‐1, a ghrelin‐releasing cell line. Further on, we characterized the ghrelin‐releasing peptides present in the digest by comprehensive peptide analysis using liquid chromatography–mass spectrometry and structure–activity relationship. Among the candidate peptides, we found that SQQQQPVLPQQPSF, LSVTSPQQVSY and YPTSL stimulated ghrelin release. We then named them wheat‐ghretropin A, B and C, respectively. In addition, we observed that wheat‐ghretropin A increased plasma ghrelin concentration and food intake in mice after oral administration. Thus, we demonstrated that wheat‐ghretropin stimulates ghrelin release both in vitro and in vivo. To the best of our knowledge, this is the first report of a wheat‐derived exogenous bioactive peptide that stimulates ghrelin secretion. By using peptide comprehensive analysis of the gluten digest and by peptide homology comparison with already known ghrelin‐releasing peptides, we discovered three novel peptides that we name wheat‐ghretropins A, B and C. We observed that wheat‐ghretropin A increased food intake and serum ghrelin levels after oral administration in mice. To the best of our knowledge, this is the first report of a wheat‐derived exogenous bioactive peptide that stimulates ghrelin secretion.
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