Extensive in vivo self-renewal, long-term reconstitution capacity, and hematopoietic multipotency of Pax5-deficient precursor B-cell clones.
Extensive in vivo self-renewal, long-term reconstitution capacity, and hematopoietic multipotency of Pax5-deficient precursor B-cell clones.
复制标题
Pax5 缺陷型前体 B 细胞克隆具有广泛的体内自我更新、长期重建能力和造血多能性。
作者：
C. Schaniel;Marie Gottar;E. Roosnek;F. Melchers;A. Rolink
自我更新、多能性和长期重建是单个造血干细胞的定义特征。Pax 5（-/-）前体B细胞显然具有类似的特征。在这里，使用连续移植，在体外再生和生长的骨髓归巢的供体细胞发生在所有移植后，我们分析了Pax 5（-/-）前体B细胞克隆的自我更新和造血多能性的程度。此外，端粒长度和端粒酶活性在这些克隆进行了分析，在不同的时间点。到目前为止，已经连续进行了5次移植。第五次移植的克隆体在体外增殖了150多次细胞分裂，仍然用前体B细胞重新填充骨髓，并用淋巴和骨髓细胞重建这些受体。在这种广泛的增殖过程中，Pax 5（-/-）前体B细胞缩短它们的端粒，每次分裂缩短70至90个碱基对。在所有系列体内移植/体外扩增期间，它们的端粒酶活性保持在HEK 293癌细胞的3%。总之，这些数据表明Pax 5（-/-）前体B细胞克隆具有广泛的体内自我更新能力、长期重建能力和造血多能性，其端粒以正常速率缩短。
Self-renewal, pluripotency, and long-term reconstitution are defining characteristics of single hematopoietic stem cells. Pax5(-/-) precursor B cells apparently possess similar characteristics. Here, using serial transplantations, with in vitro recloning and growth of the bone marrow-homed donor cells occurring after all transplantations, we analyzed the extent of self-renewal and hematopoietic multipotency of Pax5(-/-) precursor B-cell clones. Moreover, telomere length and telomerase activity in these clones was analyzed at various time points. Thus far, 5 successive transplantations have been performed. Clones transplanted for the fifth time, which have proliferated for more than 150 cell divisions in vitro, still repopulate the bone marrow with precursor B cells and reconstitute these recipients with lymphoid and myeloid cells. During this extensive proliferation, Pax5(-/-) precursor B cells shorten their telomeres at 70 to 90 base pairs per division. Their telomerase activity remains at 3% of that of HEK293 cancer cells during all serial in vivo transplantations/in vitro expansions. Together, these data show that Pax5(-/-) precursor B-cell clones possess extensive in vivo self-renewal capacity, long-term reconstitution capacity, and hematopoietic multipotency, with their telomeres shortening at the normal rate.
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影响因子：
32.4
作者：
Morrison, SJ;Prowse, KR;Weissman, IL
通讯作者：
Weissman, IL
影响因子：
10.5
作者：
JORDAN, CT;LEMISCHKA, IR
通讯作者：
LEMISCHKA, IR
DOI：
10.1126/science.7071580
发表时间：
1982
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Reincke,U;Hannon,EC;Rosenblatt,M;Hellman,S
通讯作者：
Hellman,S
影响因子：
32.4
作者：
MORRISON, SJ;WEISSMAN, IL
通讯作者：
WEISSMAN, IL
DOI：
10.1073/pnas.89.21.10114
发表时间：
1992-11-01
影响因子：
11.1
作者：
ALLSOPP, RC;VAZIRI, H;HARLEY, CB
通讯作者：
HARLEY, CB