Utility of Claudin-3 in extracellular vesicles from human bile as biomarkers of cholangiocarcinoma.
Utility of Claudin-3 in extracellular vesicles from human bile as biomarkers of cholangiocarcinoma.
复制标题
人胆汁细胞外囊泡中Claudin-3作为胆管癌生物标志物的应用
DOI：
10.1038/s41598-021-81023-y
复制
发表时间：
2021-01-13
影响因子：
4.6
通讯作者：
Ueno Y
中科院分区：
文献类型：
--
作者：
Ikeda C;Haga H;Makino N;Inuzuka T;Kurimoto A;Ueda T;Matsuda A;Kakizaki Y;Ishizawa T;Kobayashi T;Sugahara S;Tsunoda M;Suda K;Ueno Y
细胞外囊泡（EV）从所有细胞释放。胆汁直接接触胆管肿瘤;胆汁衍生的EV可能含有高浓度的癌症生物标志物。我们对人胆汁来源的EV进行了蛋白质组学分析，并确定了胆管癌（CCA）的新生物标志物。使用超离心分离EV，并使用螯合剂乙二胺四乙酸和乙二醇四乙酸（EDEG）和磷酸盐缓冲盐水（PBS）作为溶解溶液。收集10例CCA和10例胆总管结石患者的胆汁。进行蛋白质组学分析;随后，在验证组群中使用候选生物标志物进行ELISA。从胆汁中分离的囊泡具有典型的大小和形态。观察外泌体标志物的表达。RNA在EDEG组中更丰富。EDEG组中microRNA的比例较高。EDEG的使用导致去除更多的污染物。蛋白质组学分析鉴定出166种CCA特异性蛋白质。Claudin-3的ELISA检测结果显示差异有统计学意义。诊断准确性为AUC 0.945，敏感性和特异性为87.5%。我们报告了第一次使用的EDEG在分离EV从人胆汁和蛋白质组学分析的人胆汁衍生的EV蛋白在CCA。胆汁来源EV中的Claudin-3是CCA的有用生物标志物。
Extracellular vesicles (EVs) are released from all cells. Bile directly contacts bile duct tumor; bile-derived EVs may contain high concentrations of cancer biomarkers. We performed a proteomic analysis of human bile-derived EVs and identified a novel biomarker of cholangiocarcinoma (CCA). EVs were isolated using ultracentrifugation, and chelating agents, ethylenediaminetetraacetic acid and ethylene glycol tetraacetic acid (EDEG) and phosphate buffered saline (PBS) were used as dissolution solutions. Bile was collected from 10 CCA and 10 choledocholithiasis (stones) cases. Proteomic analysis was performed; subsequently, ELISA was performed using the candidate biomarkers in a verification cohort. The vesicles isolated from bile had a typical size and morphology. The expression of exosome markers was observed. RNA was more abundant in the EDEG group. The proportion of microRNA was higher in the EDEG group. EDEG use resulted in the removal of more contaminants. Proteomic analysis identified 166 proteins as CCA-specific. ELISA for Claudin-3 revealed statistically significant difference. The diagnostic accuracy was AUC 0.945 and sensitivity and specificity were 87.5%. We report the first use of EDEG in the isolation of EVs from human bile and the proteomic analysis of human bile-derived EV-proteins in CCA. Claudin-3 in bile-derived EVs is a useful biomarker for CCA.
登录
查看更多内容
DOI：
10.1007/s00534-007-1279-5
发表时间：
2008
期刊：
JOURNAL OF HEPATO-BILIARY-PANCREATIC SURGERY
影响因子：
--
作者：
Kondo, Satoshi;Takada, Tadahiro;Miyazaki, Masaru;Miyakawa, Shuichi;Tsukada, Kazuhiro;Nagino, Masato;Furuse, Junji;Saito, Hiroya;Tsuyuguchi, Toshio;Yamamoto, Masakazu;Kayahara, Masato;Kimura, Fumio;Yoshitomi, Hideyuki;Nozawa, Satoshi;Yoshida, Masahiro;Wada, Keita;Hirano, Satoshi;Amano, Hodaka;Miura, Fumihiko
通讯作者：
Miura, Fumihiko
三个紧密连接相关的Maguks ZO-1，ZO-2和ZO-3与Claudins的Cooh Termini直接结合。
DOI：
10.1083/jcb.147.6.1351
发表时间：
1999-12-13
期刊：
The Journal of cell biology
影响因子：
--
作者：
Itoh M;Furuse M;Morita K;Kubota K;Saitou M;Tsukita S
通讯作者：
Tsukita S
对医疗统计信息的自由使用的易于使用的软件“ EZR”的调查。
影响因子：
4.8
作者：
Kanda Y
通讯作者：
Kanda Y
影响因子：
14.9
作者：
Okuda S;Watanabe Y;Moriya Y;Kawano S;Yamamoto T;Matsumoto M;Takami T;Kobayashi D;Araki N;Yoshizawa AC;Tabata T;Sugiyama N;Goto S;Ishihama Y
通讯作者：
Ishihama Y
影响因子：
13.5
作者：
Arbelaiz, Ander;Azkargorta, Mikel;Banales, Jesus M.
通讯作者：
Banales, Jesus M.