Initial characterization of the Streptococcus gordonii htpX gene.

Initial characterization of the Streptococcus gordonii htpX gene.
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戈登链球菌 htpX 基因的初步表征。

DOI:
10.1046/j.0902-0055.2001.00000.x
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发表时间:
2002
影响因子:
--
通讯作者:
Edwards,CA
Edwards,CA
中科院分区:
--
文献类型:
--
作者:
Vickerman,MM;Mather,NM;Minick,PE;Edwards,CA

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检查戈登链球菌染色体区域,它位于直接上游的葡糖基转移酶阳性调控决定簇trgg,发现两个开放的阅读框架。基于核苷酸序列,这些基因与单核细胞增生李斯特菌lemA基因(参与抗原呈递)和大肠杆菌htpX热休克基因(功能未知)相似。北方杂交分析表明,S.戈登氏菌lemA和htpX基因与约1.7 kb的多顺反子转录物相关。虽然thelemA/htpX转录水平在热反应中没有增加到dnaK对照水平,但htpX的插入失活导致了在41°C下生长的细胞中的粘附、细胞形态和去污剂可提取的表面抗原的变化,这意味着htpX可能参与了表面蛋白的表达。lemA和htpX的插入失活表明,尽管它们接近gg和结构基因gtfG,但这些上游基因不影响S。戈登尼葡糖基转移酶活性。
Examination of theStreptococcus gordoniichromosomal region, which lies immediately upstream of the glucosyltransferase positive regulatory determinantrgg, revealed two open reading frames. Based on nucleotide sequences, these genes were similar to theListeria monocytogenes lemAgene, which is involved in antigen presentation, and theEscherichia coli htpXheat shock gene, which has an unknown function. Northern hybridization analysis indicated thatS. gordonii lemAandhtpXgenes were associated with aca.1.7‐kb polycistronic transcript. Although levels of thelemA/htpXtranscript did not increase in response to heat to levels seen withdnaKcontrols, insertional inactivation ofhtpXresulted in changes in adhesiveness, cellular morphology and detergent‐extractable surface antigens in cells grown at 41°C, implying thathtpXmay be involved in surface protein expression. Insertional inactivation oflemAandhtpXindicated that, despite their proximity torggand the structural gene,gtfG, these upstream genes do not affectS. gordoniiglucosyltransferase activity.
血链球菌的细胞表面蛋白与细胞疏水性和共聚集特性相关。
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