Detailed immune profiling in pediatric Crohn's disease using methylation cytometry.
Detailed immune profiling in pediatric Crohn's disease using methylation cytometry.
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DOI：
10.1080/15592294.2023.2289786
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发表时间：
2024-12
期刊：
影响因子：
3.7
通讯作者：
Christensen, Brock C.
中科院分区：
文献类型：
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作者：
Reynolds, Samuel R.;Salas, Lucas A.;Chen, Ji-Qing;Christensen, Brock C.
DNA甲基化已被广泛用于研究许多疾病的表观遗传模式以及去卷积血细胞类型比例。这项研究建立在先前研究的基础上，研究了不同阶段克罗恩病儿科患者的甲基化模式，以使用一种新的去卷积方法扩展这些患者的免疫谱。与对照组相比，我们观察到克罗恩病患者的CD4记忆细胞、幼稚细胞、CD8幼稚细胞和自然杀伤细胞水平显著降低，中性粒细胞水平升高。此外，克罗恩病患者的嗜中性粒细胞与淋巴细胞比率显著升高。使用表观基因组关联方法并调整潜在的混杂因素，包括细胞类型，我们观察到397个差异甲基化CpG（DMC）位点与克罗恩病相关。与DMC相关的最重要的遗传途径是调节精氨酸代谢过程，其参与T细胞的调节。
DNA methylation has been extensively utilized to study epigenetic patterns across many diseases as well as to deconvolve blood cell type proportions. This study builds upon previous studies examining methylation patterns in paediatric patients with varying stages of Crohn’s disease to extend the immune profiling of these patients using a novel deconvolution approach. Compared with control subjects, we observed significantly decreased levels of CD4 memory and naive, CD8 naive, and natural killer cells and elevated neutrophil levels in Crohn’s disease. In addition, Crohn’s patients had a significantly elevated neutrophil-to-lymphocyte ratio. Using an epigenome-wide association approach and adjusting for potential confounders, including cell type, we observed 397 differentially methylated CpG (DMC) sites associated with Crohn’s disease. The top genetic pathway associated with the DMCs was the regulation of arginine metabolic processes which are involved in the regulation of T cells.
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影响因子：
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作者：
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通讯作者：
Rosales C
影响因子：
24.5
作者：
Satsangi, J.;Silverberg, M. S.;Colombel, J-F
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Colombel, J-F
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作者：
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通讯作者：
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